Please use this identifier to cite or link to this item:
https://hdl.handle.net/10356/78858
Title: | Parasitic factors and gametocytogenesis | Authors: | Ang, Sheng Zhi | Keywords: | Science::Biological sciences | Issue Date: | 2019 | Abstract: | It has been shown that gametocytogenesis of malaria can be affected by environmental changes. However, the actual reason behind this occurrence is still unknown. Therefore, to study the mechanism behind this phenomenon, we have selected three possible genes that could be involved in detecting or transducing these extracellular changes to the parasite within the infected erythrocyte. These three genes include a heat shock protein 40, a serine/threonine kinase and an exported protein with unknown function. To investigate their involvement, the wildtype gene must be modified so that their expression can be controlled. In this paper, we attempted to assemble a linear plasmid via Gibson Assembly which will be later used to transfect the parasites. One of the plasmids had been successfully constructed. However, we realised that the success rate in joining the right-hand region (RHR) onto the plasmid was very low and had hypothesized that it might be due to the restriction site (I-SceI) that was used for the addition of RHR. | URI: | http://hdl.handle.net/10356/78858 | Schools: | School of Biological Sciences | Research Centres: | Singapore-MIT Alliance Programme | Rights: | Nanyang Technological University | Fulltext Permission: | restricted | Fulltext Availability: | With Fulltext |
Appears in Collections: | SBS Student Reports (FYP/IA/PA/PI) |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Ang Sheng ZHi U1640553E FYP paper final.pdf Restricted Access | 418.68 kB | Adobe PDF | View/Open |
Page view(s)
420
Updated on Mar 28, 2024
Download(s) 50
52
Updated on Mar 28, 2024
Google ScholarTM
Check
Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.