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Title: An internal ribosome entry site (IRES) mutant library for tuning expression level of multiple genes in mammalian cells
Authors: Koh, Esther Y. C.
Ho, Steven C. L.
Song, Zhiwei
Bi, Xuezhi
Bardor, Muriel
Yang, Yuansheng
Issue Date: 2013
Source: Koh, E. Y. C., Ho, S. C. L., Mariati , Song, Z., Bi, X., Bardor, M., et al. (2013). An internal ribosome entry site (IRES) mutant library for tuning expression level of multiple genes in mammalian cells. PLoS One, 8(12), e82100-.
Series/Report no.: PLoS One
Abstract: A set of mutated Encephalomyocarditis virus (EMCV) internal ribosome entry site (IRES) elements with varying strengths is generated by mutating the translation initiation codons of 10th, 11th, and 12th AUG to non-AUG triplets. They are able to control the relative expression of multiple genes over a wide range in mammalian cells in both transient and stable transfections. The relative strength of each IRES mutant remains similar in different mammalian cell lines and is not gene specific. The expressed proteins have correct molecular weights. Optimization of light chain over heavy chain expression by these IRES mutants enhances monoclonal antibody expression level and quality in stable transfections. Uses of this set of IRES mutants can be extended to other applications such as synthetic biology, investigating interactions between proteins and its complexes, cell engineering, multi-subunit protein production, gene therapy, and reprogramming of somatic cells into stem cells.
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0082100
Schools: School of Chemical and Biomedical Engineering 
Rights: © 2013 Koh et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SCBE Journal Articles

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