Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/79272
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dc.contributor.authorYang, Huanen
dc.contributor.authorShen, Yupingen
dc.contributor.authorXu, Yingen
dc.contributor.authorZheng, Jieen
dc.contributor.authorWu, Qinanen
dc.contributor.authorMaqueda, Aida Serraen
dc.contributor.authorTam, James Pingkwanen
dc.date.accessioned2015-09-22T02:42:16Zen
dc.date.accessioned2019-12-06T13:21:20Z-
dc.date.available2015-09-22T02:42:16Zen
dc.date.available2019-12-06T13:21:20Z-
dc.date.copyright2015en
dc.date.issued2015en
dc.identifier.citationYang, H., Shen, Y., Xu, Y., Maqueda, A. S., Zheng, J., Wu, Q., et al. (2015). A novel strategy for the discrimination of gelatinous Chinese medicines based on enzymatic digestion followed by nano-flow liquid chromatography in tandem with orbitrap mass spectrum detection. International Journal of Nanomedicine, 10, 4947-4955.en
dc.identifier.issn1178-2013en
dc.identifier.urihttps://hdl.handle.net/10356/79272-
dc.description.abstractGelatinous Chinese medicines made from mammalian skin or horn or reptile shell are a very important type of animal-derived Chinese medicine. They have been extensively used either as both hemopoietic and hemostatic agents to treat vertigo, palpitation, hematuria, and insomnia in traditional Chinese medicine clinics; consumed as a popular tonic for weaker persons such as the elderly or women after giving birth; or further manufactured to health supplements for certain populations. However, they cannot be discriminated from each other by only using the routine approach in the Chinese Pharmacopoeia, as it lacks enough specificity and, consequently, and the requirements can be met even by adding assayed ingredients. In this study, our efforts to differentiate three gelatinous Chinese medicines, Asini Corii Colla, Cervi Cornus Colla, and Testudinis Carapacis ET Plastri Colla, are presented, and a novel strategy based on enzymatic digestion followed by nano-flow liquid chromatography in tandem with orbitrap mass spectrum detector analysis is proposed herein. Fourteen diagnostic fragments identified from the digests of these medicines were exclusively selected for their discrimination. By taking advantage of the favorable features of this strategy, it is feasible and convenient to identify enzymatic-digested peptides originated from signature proteins in each medicine, which thus could be employed as potential biomarkers for their form of raw medicinal material, and the pulverized and the complex especially, that being the direct basis for authentication purpose.en
dc.language.isoenen
dc.relation.ispartofseriesInternational Journal of Nanomedicineen
dc.rights© 2015 Yang et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License. The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. Permissions beyond the scope of the License are administered by Dove Medical Press Limited. Information on how to request permission may be found at: http://www.dovepress.com/permissions.phpen
dc.titleA novel strategy for the discrimination of gelatinous Chinese medicines based on enzymatic digestion followed by nano-flow liquid chromatography in tandem with orbitrap mass spectrum detectionen
dc.typeJournal Articleen
dc.contributor.schoolSchool of Biological Sciencesen
dc.identifier.doi10.2147/IJN.S82291en
dc.description.versionPublished versionen
dc.identifier.pmid26345994-
item.fulltextWith Fulltext-
item.grantfulltextopen-
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