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Title: Near-infrared squaraine dye encapsulated micelles for in vivo fluorescence and photoacoustic bimodal imaging
Authors: Sreejith, Sivaramapanicker
Joseph, James
Lin, Manjing
Menon, Nishanth Venugopal
Borah, Parijat
Ng, Hao Jun
Loong, Yun Xian
Kang, Yuejun
Yu, Sidney Wing-Kwong
Zhao, Yanli
Keywords: DRNTU::Science::Chemistry
Issue Date: 2015
Source: Sreejith, S., Joseph, J., Lin, M., Menon, N. V., Borah, P., Ng, H. J., et al. (2015). Near-infrared squaraine dye encapsulated micelles for in vivo fluorescence and photoacoustic bimodal imaging. ACS Nano, 9(6), 5695-5704.
Series/Report no.: ACS Nano
Abstract: Combined near-infrared (NIR) fluorescence and photoacoustic imaging techniques present promising capabilities for noninvasive visualization of biological structures. Development of bimodal noninvasive optical imaging approaches by combining NIR fluorescence and photoacoustic tomography demands suitable NIR-active exogenous contrast agents. If the aggregation and photobleaching are prevented, squaraine dyes are ideal candidates for fluorescence and photoacoustic imaging. Herein, we report rational selection, preparation, and micelle encapsulation of an NIR-absorbing squaraine dye (D1) for in vivo fluorescence and photoacoustic bimodal imaging. D1 was encapsulated inside micelles constructed from a biocompatible nonionic surfactant (Pluoronic F-127) to obtain D1-encapsulated micelles (D1micelle) in aqueous conditions. The micelle encapsulation retains both the photophysical features and chemical stability of D1. D1micelle exhibits high photostability and low cytotoxicity in biological conditions. Unique properties of D1micelle in the NIR window of 800–900 nm enable the development of a squaraine-based exogenous contrast agent for fluorescence and photoacoustic bimodal imaging above 820 nm. In vivo imaging using D1micelle, as demonstrated by fluorescence and photoacoustic tomography experiments in live mice, shows contrast-enhanced deep tissue imaging capability. The usage of D1micelle proven by preclinical experiments in rodents reveals its excellent applicability for NIR fluorescence and photoacoustic bimodal imaging.
DOI: 10.1021/acsnano.5b02172
Schools: School of Physical and Mathematical Sciences 
School of Chemical and Biomedical Engineering 
School of Materials Science & Engineering 
Rights: © 2015 American Chemical Society. This is the author created version of a work that has been peer reviewed and accepted for publication by ACS Nano, American Chemical Society. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [].
Fulltext Permission: open
Fulltext Availability: With Fulltext
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