Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/79335
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dc.contributor.authorIyengar, Prasanna Vasudevanen
dc.contributor.authorJaynes, Patricken
dc.contributor.authorRodon, Lauraen
dc.contributor.authorLama, Dilrajen
dc.contributor.authorLaw, Kai Pongen
dc.contributor.authorLim, Yoon Pinen
dc.contributor.authorVerma, Chandraen
dc.contributor.authorSeoane, Joanen
dc.contributor.authorEichhorn, Pieter Johan Adamen
dc.date.accessioned2015-10-23T07:59:09Zen
dc.date.accessioned2019-12-06T13:22:49Z-
dc.date.available2015-10-23T07:59:09Zen
dc.date.available2019-12-06T13:22:49Z-
dc.date.copyright2015en
dc.date.issued2015en
dc.identifier.citationIyengar, P. V., Jaynes, P., Rodon, L., Lama, D., Law, K. P., Lim, Y. P., et al. (2015). USP15 regulates SMURF2 kinetics through C-lobe mediated deubiquitination. Scientific Reports, 5, 14733-.en
dc.identifier.issn2045-2322en
dc.identifier.urihttps://hdl.handle.net/10356/79335-
dc.description.abstractUbiquitin modification of the TGF-β pathway components is emerging as a key mechanism of TGF-β pathway regulation. To limit TGF-β responses, TGF-β signaling is regulated through a negative feedback loop whereby the E3 ligase SMURF2 targets the TGF-β receptor (TβR) complex for ubiquitin-mediated degradation. Counteracting this process, a number of deubiquitinating (DUBs) enzymes have recently been identified that deubiquitinate and stabilize the TβR. However the precise mechanism by which these DUBs act on TβR function remains poorly defined. Here, we demonstrate that apart from targeting the TβR complex directly, USP15 also deubiquitinates SMURF2 resulting in enhanced TβR stability and downstream pathway activation. Through proteomic analysis, we show that USP15 modulates the ubiquitination of Lys734, a residue required for SMURF2 catalytic activity. Our results show that SMURF2 is a critical target of USP15 in the TGF-β pathway and may also explain how USP15 and SMURF2 target multiple complementary protein complexes in other pathways.en
dc.language.isoenen
dc.relation.ispartofseriesScientific Reportsen
dc.rightsThis work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/en
dc.titleUSP15 regulates SMURF2 kinetics through C-lobe mediated deubiquitinationen
dc.typeJournal Articleen
dc.contributor.schoolSchool of Biological Sciencesen
dc.identifier.doi10.1038/srep14733en
dc.description.versionAccepted versionen
dc.identifier.pmid26435193-
item.fulltextWith Fulltext-
item.grantfulltextopen-
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