Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/79343
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dc.contributor.authorToh, Hean Ch'ngen
dc.contributor.authorDeSalvo, Martinaen
dc.contributor.authorLin, Peteren
dc.contributor.authorVashisht, Ajayen
dc.contributor.authorWohlschlegel, James A.en
dc.contributor.authorMartin, Kelsey C.en
dc.date.accessioned2015-09-23T06:41:03Zen
dc.date.accessioned2019-12-06T13:23:00Z-
dc.date.available2015-09-23T06:41:03Zen
dc.date.available2019-12-06T13:23:00Z-
dc.date.copyright2015en
dc.date.issued2015en
dc.identifier.citationToh, H. C., DeSalvo, M., Lin, P., Vashisht, A., Wohlschlegel, J. A., & Martin, K. C. (2015). Cell biological mechanisms of activity-dependent synapse to nucleus translocation of CRTC1 in neurons. Frontiers in Molecular Neuroscience, 8, 48-.en
dc.identifier.issn1662-5099en
dc.identifier.urihttps://hdl.handle.net/10356/79343-
dc.description.abstractPrevious studies have revealed a critical role for CREB-regulated transcriptional coactivator (CRTC1) in regulating neuronal gene expression during learning and memory. CRTC1 localizes to synapses but undergoes activity-dependent nuclear translocation to regulate the transcription of CREB target genes. Here we investigate the long-distance retrograde transport of CRTC1 in hippocampal neurons. We show that local elevations in calcium, triggered by activation of glutamate receptors and L-type voltage-gated calcium channels, initiate active, dynein-mediated retrograde transport of CRTC1 along microtubules. We identify a nuclear localization signal within CRTC1, and characterize three conserved serine residues whose dephosphorylation is required for nuclear import. Domain analysis reveals that the amino-terminal third of CRTC1 contains all of the signals required for regulated nucleocytoplasmic trafficking. We fuse this region to Dendra2 to generate a reporter construct and perform live-cell imaging coupled with local uncaging of glutamate and photoconversion to characterize the dynamics of stimulus-induced retrograde transport and nuclear accumulation.en
dc.language.isoenen
dc.relation.ispartofseriesFrontiers in Molecular Neuroscienceen
dc.rightsCopyright © 2015 Ch’ng, DeSalvo, Lin, Vashisht, Wohlschlegel and Martin. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.en
dc.titleCell biological mechanisms of activity-dependent synapse to nucleus translocation of CRTC1 in neuronsen
dc.typeJournal Articleen
dc.contributor.schoolLee Kong Chian School of Medicine (LKCMedicine)en
dc.identifier.doi10.3389/fnmol.2015.00048en
dc.description.versionPublished versionen
item.grantfulltextopen-
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Appears in Collections:LKCMedicine Journal Articles

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