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dc.contributor.authorMoser, Clausen
dc.contributor.authorKragh, Kasper N.en
dc.contributor.authorAlhede, Mortenen
dc.contributor.authorJensen, Peter Ø.en
dc.contributor.authorScheike, Thomasen
dc.contributor.authorJacobsen, Carsten S.en
dc.contributor.authorPoulsen, Steen Seieren
dc.contributor.authorEickhardt-Sørensen, Steffen Roberten
dc.contributor.authorTrøstrup, Hannahen
dc.contributor.authorChristoffersen, Larsen
dc.contributor.authorHougen, Hans-Petteren
dc.contributor.authorRickelt, Lars F.en
dc.contributor.authorKühl, Michaelen
dc.contributor.authorHøiby, Nielsen
dc.contributor.authorBjarnsholt, Thomasen
dc.identifier.citationKragh, K. N., Alhede, M., Jensen, P. Ø., Moser, C., Scheike, T., Jacobsen, C. S., et al. (2014). Polymorphonuclear leukocytes restrict growth of pseudomonas aeruginosa in the lungs of cystic fibrosis patients. Infection and immunity, 82(11), 4477-4486.en
dc.description.abstractCystic fibrosis (CF) patients have increased susceptibility to chronic lung infections by Pseudomonas aeruginosa, but the ecophysiology within the CF lung during infections is poorly understood. The aim of this study was to elucidate the in vivo growth physiology of P. aeruginosa within lungs of chronically infected CF patients. A novel, quantitative peptide nucleic acid (PNA) fluorescence in situ hybridization (PNA-FISH)-based method was used to estimate the in vivo growth rates of P. aeruginosa directly in lung tissue samples from CF patients and the growth rates of P. aeruginosa in infected lungs in a mouse model. The growth rate of P. aeruginosa within CF lungs did not correlate with the dimensions of bacterial aggregates but showed an inverse correlation to the concentration of polymorphonuclear leukocytes (PMNs) surrounding the bacteria. A growth-limiting effect on P. aeruginosa by PMNs was also observed in vitro, where this limitation was alleviated in the presence of the alternative electron acceptor nitrate. The finding that P. aeruginosa growth patterns correlate with the number of surrounding PMNs points to a bacteriostatic effect by PMNs via their strong O2 consumption, which slows the growth of P. aeruginosa in infected CF lungs. In support of this, the growth of P. aeruginosa was significantly higher in the respiratory airways than in the conducting airways of mice. These results indicate a complex host-pathogen interaction in chronic P. aeruginosa infection of the CF lung whereby PMNs slow the growth of the bacteria and render them less susceptible to antibiotic treatment while enabling them to persist by anaerobic respiration.en
dc.relation.ispartofseriesInfection and immunityen
dc.rights© 2014 American Society for Microbiology. This paper was published in Infection and Immunity and is made available as an electronic reprint (preprint) with permission of American Society for Microbiology. The paper can be found at the following official DOI: []. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law.en
dc.subjectDRNTU::Science::Biological sciences::Microbiology::Immunologyen
dc.titlePolymorphonuclear leukocytes restrict growth of pseudomonas aeruginosa in the lungs of cystic fibrosis patientsen
dc.typeJournal Articleen
dc.contributor.schoolSchool of Biological Sciencesen
dc.contributor.organizationSingapore Centre for Environmental Life Sciences Engineeringen
dc.description.versionPublished versionen
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