Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/79448
Title: Time-resolved botulinum neurotoxin A activity monitored using peptide-functionalized Au nanoparticle energy transfer sensors
Authors: Wang, Yi
Liu, Xiaohu
Zhang, Jinling
Aili, Daniel
Liedberg, Bo
Keywords: Energy Transfer
Gold
DRNTU::Engineering::Nanotechnology
Issue Date: 2014
Source: Wang, Y., Liu, X., Zhang, J., Aili, D., & Liedberg, B. (2014). Time-resolved botulinum neurotoxin A activity monitored using peptide-functionalized Au nanoparticle energy transfer sensors. Chemical Science, 5(7), 2651-2656.
Series/Report no.: Chemical Science
Abstract: We report herein on the employment of synthetic peptide-functionalized gold nanoparticles (AuNPs) with various diameters as radiative quenchers for the time-resolved monitoring of botulinum A light chain (BoLcA) activity. The results demonstrate that larger AuNPs provide higher energy transfer efficiencies between the dye and the AuNPs, but poorer BoLcA activities for the proteolysis of peptides because of steric constraints. The initial turnover number for the BoLcA proteolysis of peptides on 18 nm AuNPs was retarded by a factor of 80 as compared with 1.4 nm AuNPs. A similar phenomenon has been observed for trypsin, however, with less hindrance on large AuNPs. Thus, the use of smaller 1.4 nm AuNPs in conjunction with robust synthetic peptides provides an attractive format for the time-resolved monitoring of protease activity and for BoLcA sensing at a highly competitive limit of detection (1 pM).
URI: https://hdl.handle.net/10356/79448
http://hdl.handle.net/10220/38490
DOI: 10.1039/c3sc53305k
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:MSE Journal Articles

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