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Title: Nitric oxide treatment for the control of reverse osmosis membrane biofouling
Authors: Fane, Anthony G.
Kjelleberg, Staffan
Rice, Scott A.
Barnes, Robert J.
Low, Jiun Hui
Bandi, Ratnaharika R.
Tay, Martin
Chua, Felicia
Aung, Theingi
Keywords: DRNTU::Science::Biological sciences::Microbiology
Issue Date: 2015
Source: Barnes, R. J., Low, J. H., Bandi, R. R., Tay, M., Chua, F., Aung, T., et al. (2015). Nitric oxide treatment for the control of reverse osmosis membrane biofouling. Applied and environmental microbiology, 81(7), 2515-2524.
Series/Report no.: Applied and environmental microbiology
Abstract: Biofouling remains a key challenge for membrane-based water treatment systems. This study investigated the dispersal potential of the nitric oxide (NO) donor compound, PROLI NONOate, on single- and mixed-species biofilms formed by bacteria isolated from industrial membrane bioreactor and reverse osmosis (RO) membranes. The potential of PROLI NONOate to control RO membrane biofouling was also examined. Confocal microscopy revealed that PROLI NONOate exposure induced biofilm dispersal in all but two of the bacteria tested and successfully dispersed mixed-species biofilms. The addition of 40 μM PROLI NONOate at 24-h intervals to a laboratory-scale RO system led to a 92% reduction in the rate of biofouling (pressure rise over a given period) by a bacterial community cultured from an industrial RO membrane. Confocal microscopy and extracellular polymeric substances (EPS) extraction revealed that PROLI NONOate treatment led to a 48% reduction in polysaccharides, a 66% reduction in proteins, and a 29% reduction in microbial cells compared to the untreated control. A reduction in biofilm surface coverage (59% compared to 98%, treated compared to control) and average thickness (20 μm compared to 26 μm, treated compared to control) was also observed. The addition of PROLI NONOate led to a 22% increase in the time required for the RO module to reach its maximum transmembrane pressure (TMP), further indicating that NO treatment delayed fouling. Pyrosequencing analysis revealed that the NO treatment did not significantly alter the microbial community composition of the membrane biofilm. These results present strong evidence for the application of PROLI NONOate for prevention of RO biofouling.
DOI: 10.1128/AEM.03404-14
Rights: © 2015 American Society for Microbiology. This paper was published in Applied and Environmental Microbiology and is made available as an electronic reprint (preprint) with permission of American Society for Microbiology. The paper can be found at the following official DOI: []. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law.
Fulltext Permission: open
Fulltext Availability: With Fulltext
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