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Title: Intracellular delivery of antisense peptide nucleic acid by fluorescent mesoporous silica nanoparticles
Authors: Ma, Xing
Qu, Qiuyu
Devi, Gitali
Toh, Desiree-Faye Kaixin
Chen, Gang
Zhao, Yanli
Keywords: DRNTU::Science::Chemistry::Biochemistry
Issue Date: 2014
Source: Ma, X., Devi, G., Qu, Q., Toh, D.-F. K., Chen, G., & Zhao, Y. (2014). Intracellular delivery of antisense peptide nucleic acid by fluorescent mesoporous silica nanoparticles. Bioconjugate chemistry, 25(8), 1412-1420.
Series/Report no.: Bioconjugate chemistry
Abstract: In order to overcome poor cell permeability of antisense peptide nucleic acid (PNA), a fluorescent mesoporous silica nanoparticle (MSNP) carrier was developed to successfully deliver antisense PNA into cancer cells for effective silence of B-cell lymphoma 2 (Bcl-2) protein expression in vitro. First, fluorescent MSNP functionalized with disulfide bond bridged groups was fabricated and characterized. Antisense and negative control PNAs were synthesized and further conjugated with fluorescent dye cyanine 5. Then, the PNAs were covalently connected with fluorescent MSNP via amidation between amino group of PNAs and carboxylic acid group on the MSNP surface. High intracellular concentration of glutathione serves as a natural reducing agent, which could cleave the disulfide bond to trigger the PNA release in vitro. Confocal laser scanning microscopy studies prove that PNA conjugated MSNP was endocytosed by HeLa cancer cells, and redox-controlled intracellular release of antisense PNA from fluorescent MSNP was successfully achieved. Finally, effective silencing of the Bcl-2 protein expression induced by the delivered antisense PNA into HeLa cells was confirmed by Western blot assay.
DOI: 10.1021/bc5002714
Schools: School of Materials Science & Engineering 
School of Physical and Mathematical Sciences 
Rights: © 2014 American Chemical Society. This is the author created version of a work that has been peer reviewed and accepted for publication by Bioconjugate Chemistry, American Chemical Society. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [].
Fulltext Permission: open
Fulltext Availability: With Fulltext
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