Please use this identifier to cite or link to this item:
https://hdl.handle.net/10356/79770
Title: | Sample loading and retrieval by centrifugation in a closed-loop PCR microchip | Authors: | Lok, Khoi Seng Kwok, Yien Chian Nguyen, Nam-Trung |
Keywords: | DRNTU::Engineering::Computer science and engineering::Computer applications::Computer-aided engineering | Issue Date: | 2011 | Source: | Lok, K. S., Kwok, Y. C., & Nguyen, N. T. (2011). Sample loading and retrieval by centrifugation in a closed-loop PCR microchip. Microchimica Acta, 176, 445-453. | Series/Report no.: | Microchimica acta | Abstract: | We report on a novel concept of sample loading for microfluidic devices using a benchtop centrifuge and a magnetically actuated circular closed-loop PCR microchip as a model system. The PCR mixture and the ferrofluid were loaded into a specially designed microchip. The microchip was then placed in an off-the-shelf 50-mL tube and centrifuged. The strong centrifugal force drives the PCR mixture and the ferrofluid into the microchannels of the microchip, and simultaneously expels any trapped microbubbles. PCR was successfully carried out on single and parallel closed-loop PCR microchips. The addition of a few off-chip handling steps allows great simplification of the device design. This new loading concept may be useful for designing robust and low-cost lab-on-a-chip devices because benchtop centrifuges are quite common in most laboratories. | URI: | https://hdl.handle.net/10356/79770 http://hdl.handle.net/10220/7686 |
DOI: | 10.1007/s00604-011-0741-x | Schools: | School of Mechanical and Aerospace Engineering | Rights: | © 2011 Springer-Verlag. This is the author created version of a work that has been peer reviewed and accepted for publication in Microchimica Acta, published by Springer-Verlag. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: http://dx.doi.org/10.1007/s00604-011-0741-x. | Fulltext Permission: | open | Fulltext Availability: | With Fulltext |
Appears in Collections: | MAE Journal Articles |
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150.Sample loading and retrieval by centrifuge in closed.pdf | 450.87 kB | Adobe PDF | View/Open |
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