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dc.contributor.authorHo, Chia-Huaen
dc.contributor.authorPruksakorn, Dumnoensunen
dc.contributor.authorBabu, I. Rameshen
dc.contributor.authorNg, Chee Shengen
dc.contributor.authorHia, Fabianen
dc.contributor.authorChionh, Yok Hianen
dc.contributor.authorMcBee, Megan E.en
dc.contributor.authorSu, Danen
dc.contributor.authorPang, Yan Ling Joyen
dc.contributor.authorGu, Chenen
dc.contributor.authorDong, Hongpingen
dc.contributor.authorPrestwich, Erin G.en
dc.contributor.authorShi, Pei-Yongen
dc.contributor.authorPreiser, Peter Raineren
dc.contributor.authorAlonso, Sylvieen
dc.contributor.authorDedon, Peter C.en
dc.identifier.citationChionh, Y. H., Ho, C.-H., Pruksakorn, D., Ramesh Babu, I., Ng, C. S., Hia, F., et al. (2013). A multidimensional platform for the purification of non-coding RNA species. Nucleic acids research, 41(17), e168-.en
dc.description.abstractA renewed interest in non-coding RNA (ncRNA) has led to the discovery of novel RNA species and post-transcriptional ribonucleoside modifications, and an emerging appreciation for the role of ncRNA in RNA epigenetics. Although much can be learned by amplification-based analysis of ncRNA sequence and quantity, there is a significant need for direct analysis of RNA, which has led to numerous methods for purification of specific ncRNA molecules. However, no single method allows purification of the full range of cellular ncRNA species. To this end, we developed a multidimensional chromatographic platform to resolve, isolate and quantify all canonical ncRNAs in a single sample of cells or tissue, as well as novel ncRNA species. The applicability of the platform is demonstrated in analyses of ncRNA from bacteria, human cells and plasmodium-infected reticulocytes, as well as a viral RNA genome. Among the many potential applications of this platform are a system-level analysis of the dozens of modified ribonucleosides in ncRNA, characterization of novel long ncRNA species, enhanced detection of rare transcript variants and analysis of viral genomes.en
dc.relation.ispartofseriesNucleic Acids Researchen
dc.rights© 2013 The Author(s). Published by Oxford University Press. This paper was published in Nucleic Acids Research and is made available as an electronic reprint (preprint) with permission of the Author(s). The paper can be found at the following official DOI:  One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law.en
dc.subjectDRNTU::Science::Biological sciences::Geneticsen
dc.titleA multidimensional platform for the purification of non-coding RNA speciesen
dc.typeJournal Articleen
dc.contributor.schoolSchool of Biological Sciencesen
dc.description.versionPublished versionen
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