Please use this identifier to cite or link to this item:
Title: A multidimensional platform for the purification of non-coding RNA species
Authors: Ho, Chia-Hua
Pruksakorn, Dumnoensun
Babu, I. Ramesh
Ng, Chee Sheng
Hia, Fabian
Chionh, Yok Hian
McBee, Megan E.
Su, Dan
Pang, Yan Ling Joy
Gu, Chen
Dong, Hongping
Prestwich, Erin G.
Shi, Pei-Yong
Preiser, Peter Rainer
Alonso, Sylvie
Dedon, Peter C.
Keywords: DRNTU::Science::Biological sciences::Genetics
Issue Date: 2013
Source: Chionh, Y. H., Ho, C.-H., Pruksakorn, D., Ramesh Babu, I., Ng, C. S., Hia, F., et al. (2013). A multidimensional platform for the purification of non-coding RNA species. Nucleic acids research, 41(17), e168-.
Series/Report no.: Nucleic Acids Research
Abstract: A renewed interest in non-coding RNA (ncRNA) has led to the discovery of novel RNA species and post-transcriptional ribonucleoside modifications, and an emerging appreciation for the role of ncRNA in RNA epigenetics. Although much can be learned by amplification-based analysis of ncRNA sequence and quantity, there is a significant need for direct analysis of RNA, which has led to numerous methods for purification of specific ncRNA molecules. However, no single method allows purification of the full range of cellular ncRNA species. To this end, we developed a multidimensional chromatographic platform to resolve, isolate and quantify all canonical ncRNAs in a single sample of cells or tissue, as well as novel ncRNA species. The applicability of the platform is demonstrated in analyses of ncRNA from bacteria, human cells and plasmodium-infected reticulocytes, as well as a viral RNA genome. Among the many potential applications of this platform are a system-level analysis of the dozens of modified ribonucleosides in ncRNA, characterization of novel long ncRNA species, enhanced detection of rare transcript variants and analysis of viral genomes.
ISSN: 0305-1048
DOI: 10.1093/nar/gkt668
Schools: School of Biological Sciences 
Rights: © 2013 The Author(s). Published by Oxford University Press. This paper was published in Nucleic Acids Research and is made available as an electronic reprint (preprint) with permission of the Author(s). The paper can be found at the following official DOI:  One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SBS Journal Articles

Files in This Item:
File Description SizeFormat 
A multidimensional platform for the purification of non-coding RNA species.pdf5.15 MBAdobe PDFThumbnail

Citations 10

Updated on Sep 22, 2023

Web of ScienceTM
Citations 10

Updated on Sep 13, 2023

Page view(s) 10

Updated on Sep 21, 2023

Download(s) 5

Updated on Sep 21, 2023

Google ScholarTM




Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.