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Title: Low-dose tetracycline exposure alters gut bacterial metabolism and host-immune response : “personalized” effect?
Authors: Keerthisinghe, Tharushi Prabha
Wang, Mengjing
Zhang, Yingdan
Dong, Wu
Fang, Mingliang
Keywords: Tetracycline
Engineering::Civil engineering
Gut Bacteria
Issue Date: 2019
Source: Keerthisinghe, T. P., Wang, M., Zhang, Y., Dong, W., & Fang, M. (2019). Low-dose tetracycline exposure alters gut bacterial metabolism and host-immune response : “personalized” effect?. Environment International, 131, 104989-. doi:10.1016/j.envint.2019.104989
Series/Report no.: Environment International
Abstract: The human gut microbiome (GM) in healthy people is chronically exposed to tetracycline (TET) via environmental exposure and dietary uptake. However, limited information is available on its effect on the GM metabolome and effect on the host, especially at the dietary exposure level. Here, we investigated how TET at both sub-pharmaceutical and dietary exposure levels affects the metabolome and the secretome-induced host immune response by studying several representative gut bacteria. Interestingly, the metabolome showed a highly species-specific pattern with a distinct dose-response relationship. B. fragilis was highly sensitive to TET and vitamin, nucleotide, and amino acid metabolism pathways were the most vulnerable metabolic pathways at dietary exposure level. For key metabolite short chain fatty acids, TET significantly induced the synthesis of butyrate in B. fragilis, rather than C. sporogenes and E. coli. Furthermore, TET induced the release of lipopolysaccharides (LPS) in E. coli and enhanced the immune response; however, there was no obvious effect on B. fragilis. Interestingly, the overall immune response modulation with TET exposure relied on the ratio between E. coli and B. fragilis, possibly due to the neutralization of active LPS from E. coli by the LPS from B. fragilis. Overall, our results showed that the effect of TET from environmental exposure on the host health would be highly dependent on the GM composition, especially for the gut bacterial metabolome and secretome induced immune response.
ISSN: 0160-4120
DOI: 10.1016/j.envint.2019.104989
Schools: School of Civil and Environmental Engineering 
Research Centres: Advanced Environmental Biotechnology Centre (AEBC) 
Nanyang Environment and Water Research Institute 
Rights: © 2019 The Author(s). Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:CEE Journal Articles
NEWRI Journal Articles

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