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Title: Visualizing the perturbation of cellular cyclic di-GMP levels in bacterial cells
Authors: Ho, Chun Loong
Chong, Kavin Shi Jie
Oppong, Jamila Akosua
Chuah, Mary Lay-Cheng
Tan, Suet Mien
Liang, Zhao-Xun
Keywords: DRNTU::Science::Biological sciences
Issue Date: 2013
Source: Ho, C. L., Chong, K. S. J., Oppong, J. A., Chuah, M. L. C., Tan, S. M., & Liang, Z. X. (2013). Visualizing the Perturbation of Cellular Cyclic di-GMP Levels in Bacterial Cells. Journal of the American Chemical Society, 135(2), 566-569.
Series/Report no.: Journal of the American chemical society
Abstract: Cyclic di-GMP (c-di-GMP) has emerged as a prominent intracellular messenger that coordinates biofilm formation and pathogenicity in many bacterial species. Developing genetically encoded biosensors for c-di-GMP will help us understand how bacterial cells respond to environmental changes via the modulation of cellular c-di-GMP levels. Here we report the design of two genetically encoded c-di-GMP fluorescent biosensors with complementary dynamic ranges. By using the biosensors, we found that several compounds known to promote biofilm dispersal trigger a decline in c-di-GMP levels in Escherichia coli cells. In contrast, cellular c-di-GMP levels were elevated when the bacterial cells were treated with subinhibitory concentrations of biofilm-promoting antibiotics. The biosensors also revealed that E. coli cells engulfed by macrophages exhibit lower c-di-GMP levels, most likely as a response to the enormous pressures of survival during phagocytosis.
DOI: 10.1021/ja310497x
Schools: School of Biological Sciences 
Rights: © 2013 American Chemical Society. This is the author created version of a work that has been peer reviewed and accepted for publication by Journal of the American Chemical Society, American Chemical Society. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [].
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SBS Journal Articles

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