Please use this identifier to cite or link to this item:
https://hdl.handle.net/10356/79909
Title: | Visualizing the perturbation of cellular cyclic di-GMP levels in bacterial cells | Authors: | Ho, Chun Loong Chong, Kavin Shi Jie Oppong, Jamila Akosua Chuah, Mary Lay-Cheng Tan, Suet Mien Liang, Zhao-Xun |
Keywords: | DRNTU::Science::Biological sciences | Issue Date: | 2013 | Source: | Ho, C. L., Chong, K. S. J., Oppong, J. A., Chuah, M. L. C., Tan, S. M., & Liang, Z. X. (2013). Visualizing the Perturbation of Cellular Cyclic di-GMP Levels in Bacterial Cells. Journal of the American Chemical Society, 135(2), 566-569. | Series/Report no.: | Journal of the American chemical society | Abstract: | Cyclic di-GMP (c-di-GMP) has emerged as a prominent intracellular messenger that coordinates biofilm formation and pathogenicity in many bacterial species. Developing genetically encoded biosensors for c-di-GMP will help us understand how bacterial cells respond to environmental changes via the modulation of cellular c-di-GMP levels. Here we report the design of two genetically encoded c-di-GMP fluorescent biosensors with complementary dynamic ranges. By using the biosensors, we found that several compounds known to promote biofilm dispersal trigger a decline in c-di-GMP levels in Escherichia coli cells. In contrast, cellular c-di-GMP levels were elevated when the bacterial cells were treated with subinhibitory concentrations of biofilm-promoting antibiotics. The biosensors also revealed that E. coli cells engulfed by macrophages exhibit lower c-di-GMP levels, most likely as a response to the enormous pressures of survival during phagocytosis. | URI: | https://hdl.handle.net/10356/79909 http://hdl.handle.net/10220/9326 |
DOI: | 10.1021/ja310497x | Schools: | School of Biological Sciences | Rights: | © 2013 American Chemical Society. This is the author created version of a work that has been peer reviewed and accepted for publication by Journal of the American Chemical Society, American Chemical Society. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1021/ja310497x]. | Fulltext Permission: | open | Fulltext Availability: | With Fulltext |
Appears in Collections: | SBS Journal Articles |
Files in This Item:
File | Description | Size | Format | |
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3. Visualizing the Perturbation of Cellular Cyclic di-GMP Levels in Bacterial Cells (part1).pdf | 622.5 kB | Adobe PDF | View/Open | |
3. Visualizing the Perturbation of Cellular Cyclic di-GMP Levels in Bacterial Cells (part2).pdf | 802.54 kB | Adobe PDF | View/Open |
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