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|Title:||Adhesion, proliferation, and gene expression profile of human umbilical vein endothelial cells cultured on bilayered polyelectrolyte coatings composed of glycosaminoglycans||Authors:||Boey, Freddy Yin Chiang
Heng, Boon Chin
Bezerra, Paula Porto
Meng, Qing Rui
Chin, Desmond Wai-Loon
Koh, Li Buay
Preiser, Peter Rainer
Venkatraman, Subbu S.
|Keywords:||DRNTU::Science::Medicine::Biomedical engineering||Issue Date:||2010||Source:||Heng, B. C., Bezerra, P. P., Meng, Q. R., Chin, D. W. L., Koh, L. B., Li, H., et al. (2010). Adhesion, proliferation, and gene expression profile of human umbilical vein endothelial cells cultured on bilayered polyelectrolyte coatings composed of glycosaminoglycans. Biointerphases, 5(3).||Series/Report no.:||Biointerphases||Abstract:||This study characterized human umbilical vein endothelial cell HUVEC adhesion, proliferation, and gene expression on bilayered polyelectrolyte coatings composed of an outermost layer of glycosaminoglycans hyaluronan, heparin, or chondroitin sulfate, with an underlying layer of poly-l-lysine or chitosan. The proportion of cells that adhered to the various polyelectrolyte coatings after 1 and 2 h incubations was quantified by the WST-8 assay. Interchanging poly-l-lysine with chitosan resulted in significant differences in cellular adhesion to the outermost glycosaminoglycan layer after 1 h, but these differences became insignificant after 2 h. The proliferation of HUVEC on the various bilayered polyelectrolyte coatings over 10 days was characterized using the WST-8 assay. Regardless of whether the underlying layer was poly-l-lysine or chitosan, HUVEC proliferation on the hyaluronan outermost layer was significantly less than on heparin or chondroitin sulfate. Additionally, it was observed that there was more proliferation with poly-l-lysine as the underlying layer, compared to chitosan. Subsequently, real-time polymerase chain reaction was used to analyze the expression of seven genes related to adhesion, migration, and endothelial function (VWF, VEGFR, VEGFA, endoglin, integrin-α5, ICAM1, and ICAM2 by HUVEC cultured on the various bilayered polyelectrolyte coatings for 3 days. With poly-l-lysine as the underlying layer, biologically significant differences greater than twofold in the expression of VWF, VEGFR, VEGFA, endoglin, and ICAM1 were observed among the three glycosaminoglycans. With chitosan as the underlying layer, all three glycosaminoglycans displayed biologically significant differences in the expression of VWF and VEGFR compared to the chitosan control. CT-HA displayed the highest level of expression of VWF, whereas expression levels of VEGFR were almost similar among the three glycosaminoglycans.||URI:||https://hdl.handle.net/10356/79974
|ISSN:||1934-8630||DOI:||10.1116/1.3483218||Rights:||© 2010 American Vacuum Society This paper was published in Biointerphases and is made available as an electronic reprint (preprint) with permission of American Vacuum Society. The paper can be found at the following official URL: [http://dx.doi.org/10.1116/1.3483218 ]. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law.||Fulltext Permission:||open||Fulltext Availability:||With Fulltext|
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