Please use this identifier to cite or link to this item:
https://hdl.handle.net/10356/80218
Title: | Using amino-labeled nucleotide probes for simultaneous single molecule RNA-DNA FISH | Authors: | Basu, Reelina Lai, Lan-Tian Meng, Zhenyu Wu, Jun Shao, Fangwei Zhang, Li-Feng |
Keywords: | RNA and DNA FISH | Issue Date: | 2014 | Source: | Basu, R., Lai, L. T., Meng, Z., Wu, J., Shao, F., & Zhang, L. F. (2014). Using amino-labeled nucleotide probes for simultaneous single molecule RNA-DNA FISH. PLoS ONE, 9(9), e107425-. | Series/Report no.: | PLoS ONE | Abstract: | Using amino-labeled oligonucleotide probes, we established a simple, robust and low-noise method for simultaneous detection of RNA and DNA by fluorescence in situ hybridization, a highly useful tool to study the large pool of long noncoding RNAs being identified in the current research. With probes either chemically or biologically synthesized, we demonstrate that the method can be applied to study a wide range of RNA and DNA targets at the single-cell and singlemolecule level in cellular contexts. | URI: | https://hdl.handle.net/10356/80218 http://hdl.handle.net/10220/38859 |
ISSN: | 1932-6203 | DOI: | 10.1371/journal.pone.0107425 | Schools: | School of Biological Sciences School of Physical and Mathematical Sciences |
Rights: | © 2014 Basu et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | Fulltext Permission: | open | Fulltext Availability: | With Fulltext |
Appears in Collections: | SBS Journal Articles SPMS Journal Articles |
Files in This Item:
File | Description | Size | Format | |
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journal.pone.0107425.pdf | 1.42 MB | Adobe PDF | View/Open |
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