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Title: Using amino-labeled nucleotide probes for simultaneous single molecule RNA-DNA FISH
Authors: Basu, Reelina
Lai, Lan-Tian
Meng, Zhenyu
Wu, Jun
Shao, Fangwei
Zhang, Li-Feng
Keywords: RNA and DNA FISH
Issue Date: 2014
Source: Basu, R., Lai, L. T., Meng, Z., Wu, J., Shao, F., & Zhang, L. F. (2014). Using amino-labeled nucleotide probes for simultaneous single molecule RNA-DNA FISH. PLoS ONE, 9(9), e107425-.
Series/Report no.: PLoS ONE
Abstract: Using amino-labeled oligonucleotide probes, we established a simple, robust and low-noise method for simultaneous detection of RNA and DNA by fluorescence in situ hybridization, a highly useful tool to study the large pool of long noncoding RNAs being identified in the current research. With probes either chemically or biologically synthesized, we demonstrate that the method can be applied to study a wide range of RNA and DNA targets at the single-cell and singlemolecule level in cellular contexts.
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0107425
Schools: School of Biological Sciences 
School of Physical and Mathematical Sciences 
Rights: © 2014 Basu et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SBS Journal Articles
SPMS Journal Articles

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