Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/80260
Full metadata record
DC FieldValueLanguage
dc.contributor.authorTissera, Hasithaen
dc.contributor.authorAmarasinghe, Anandaen
dc.contributor.authorGunasena, Sunethraen
dc.contributor.authorDeSilva, Aruna Dharshanen
dc.contributor.authorYee, Leong Weien
dc.contributor.authorSessions, Octoberen
dc.contributor.authorMuthukuda, Chanakaen
dc.contributor.authorPalihawadana, Pabaen
dc.contributor.authorLohr, Wolfgangen
dc.contributor.authorByass, Peteren
dc.contributor.authorGubler, Duane J.en
dc.contributor.authorWilder-Smith, Anneliesen
dc.contributor.editorBingham, Andreaen
dc.date.accessioned2016-04-12T09:20:43Zen
dc.date.accessioned2019-12-06T13:46:05Z-
dc.date.available2016-04-12T09:20:43Zen
dc.date.available2019-12-06T13:46:05Z-
dc.date.issued2016en
dc.identifier.citationTissera, H., Amarasinghe, A., Gunasena, S., DeSilva, A. D., Yee, L. W., Sessions, O., et al. (2016). Laboratory-Enhanced Dengue Sentinel Surveillance in Colombo District, Sri Lanka: 2012-2014. PLOS Neglected Tropical Diseases, 10(2), e0004477-.en
dc.identifier.issn1935-2727en
dc.identifier.urihttps://hdl.handle.net/10356/80260-
dc.description.abstractIntroduction: Dengue has emerged as a significant public health problem in Sri Lanka. Historically surveillance was passive, with mandatory dengue notifications based on clinical diagnosis with only limited laboratory confirmation. To obtain more accurate data on the disease burden of dengue, we set up a laboratory-based enhanced sentinel surveillance system in Colombo District. Here we describe the study design and report our findings of enhanced surveillance in the years 2012–2014. Methods: Three outpatient clinics and three government hospitals in Colombo District that covered most of the Colombo metropolitan area were selected for the sentinel surveillance system. Up to 60 patients per week presenting with an undifferentiated fever were enrolled. Acute blood samples from each patient were tested by dengue specific PCR, NS1 ELISA and IgM ELISA. A sub-set of samples was sent to Duke-NUS Singapore for quality assurance, virus isolation and serotyping. Trained medical research assistants used a standardized case report form to record clinical and epidemiological data. Clinical diagnoses by the clinicians-in-charge were recorded for hospitalized cases. Results: Of 3,127 febrile cases, 43.6% were PCR and/or NS1 positive for dengue. A high proportion of lab confirmed dengue was observed from inpatients (IPD) (53.9%) compared to outpatient (clinics in hospitals and general practice) (7.6%). Dengue hemorrhagic fever (DHF) was diagnosed in 11% of patients at the time of first contact, and the median day of illness at time of presentation to the sentinel sites was 4. Dengue serotype 1 was responsible for 85% of the cases and serotype 4 for 15%. The sensitivity and specificity of the clinicians’ presumptive diagnosis of dengue was 84% and 34%, respectively. Conclusion: DENV-1, and to a lesser degree DENV-4, infection were responsible for a high proportion of febrile illnesses in Colombo in the years 2012 to 2014. Clinicians’ diagnoses were associated with high sensitivity, but laboratory confirmation is required to enhance specificity.en
dc.format.extent14 p.en
dc.language.isoenen
dc.relation.ispartofseriesPLOS Neglected Tropical Diseasesen
dc.rights© 2016 Tissera et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en
dc.subjectSri Langkaen
dc.subjectInfectious disease surveillanceen
dc.subjectDiagnostic medicineen
dc.subjectGovernment Laboratoriesen
dc.subjectClinical laboratoriesen
dc.subjectDisease surveillanceen
dc.subjectDengue feveren
dc.subjectResearch laboratoriesen
dc.titleLaboratory-Enhanced Dengue Sentinel Surveillance in Colombo District, Sri Lanka: 2012-2014en
dc.typeJournal Articleen
dc.contributor.schoolLee Kong Chian School of Medicine (LKCMedicine)en
dc.identifier.doi10.1371/journal.pntd.0004477en
dc.description.versionPublished versionen
dc.identifier.pmid26927901-
item.fulltextWith Fulltext-
item.grantfulltextopen-
Appears in Collections:LKCMedicine Journal Articles
Files in This Item:
File Description SizeFormat 
Laboratory-Enhanced Dengue Sentinel Surveillance in Colombo District, Sri Lanka.PDF880.48 kBAdobe PDFThumbnail
View/Open

SCOPUSTM   
Citations 20

24
Updated on Jul 13, 2024

Web of ScienceTM
Citations 10

26
Updated on Oct 29, 2023

Page view(s) 20

720
Updated on Jul 13, 2024

Download(s) 20

183
Updated on Jul 13, 2024

Google ScholarTM

Check

Altmetric


Plumx

Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.