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dc.contributor.authorHu, Cheng Chengen
dc.contributor.authorGiannis, Apostolosen
dc.contributor.authorChen, Chia-Lungen
dc.contributor.authorQi, Weien
dc.contributor.authorWang, Jing-Yuanen
dc.identifier.citationHu, C. C., Giannis, A., Chen, C. L., Qi, W., & Wang, J. Y. (2013). Comparative study of biohydrogen production by four dark fermentative bacteria. International Journal of Hydrogen Energy, 38(35), 15686-15692.en
dc.description.abstractDark fermentation is a promising biological method for hydrogen production because of its high production rate in the absence of light source and variety of the substrates. In this study, hydrogen production potential of four dark fermentative bacteria (Clostridium butyricum, Clostridium pasteurianum, Clostridium beijerinckii, and Enterobacter aerogenes) using glucose as substrate was investigated under anaerobic conditions. Batch experiments were conducted to study the effects of initial glucose concentration on hydrogen yield, hydrogen production rate and concentration of volatile fatty acids (VFA) in the effluents. Among the four different fermentative bacteria, C. butyricum showed great performance at 10 g/L of glucose with hydrogen production rate of 18.29 mL-H2/L-medium/hand specific hydrogen production rate of 3.90 mL-H2/g-biomass/h. In addition, it was found that the distribution of volatile fatty acids was different among the fermentative bacteria. C. butyricum and C. pasteurianum had higher ratio of acetate to butyrate compared to the other two species, which favored hydrogen generation.en
dc.relation.ispartofseriesInternational Journal of Hydrogen Energyen
dc.rights© 2013 Hydrogen Energy Publications, LLC. Published by Elsevier Ltden
dc.subjectClostridium butyricumen
dc.subjectClostridium pasteurianumen
dc.subjectEnterobacter aerogenesen
dc.subjectClostridium beijerinckiien
dc.subjectDark fermentative bacteriaen
dc.titleComparative study of biohydrogen production by four dark fermentative bacteriaen
dc.typeJournal Articleen
dc.contributor.schoolSchool of Civil and Environmental Engineeringen
dc.contributor.researchNanyang Environment and Water Research Instituteen
dc.contributor.researchResidues and Resource Reclamation Centreen
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