Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/80456
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dc.contributor.authorGandhimathi, Chinnasamyen
dc.contributor.authorPraveena, Jayaramanen
dc.contributor.authorRamakrishna, Seeramen
dc.contributor.authorTham, Allister Yingweien
dc.contributor.authorVenugopal, Jayarama Reddyen
dc.contributor.authorKumar, Srinivasan Dineshen
dc.date.accessioned2018-11-02T02:13:45Zen
dc.date.accessioned2019-12-06T13:49:54Z-
dc.date.available2018-11-02T02:13:45Zen
dc.date.available2019-12-06T13:49:54Z-
dc.date.issued2016en
dc.identifier.citationTham, A. Y., Gandhimathi, C., Praveena, J., Venugopal, J. R., Ramakrishna, S., & Kumar, S. D. (2016). Minocycline loaded hybrid composites nanoparticles for mesenchymal stem cells differentiation into osteogenesis. International Journal of Molecular Sciences, 17(8), 1222-. doi:10.3390/ijms17081222en
dc.identifier.issn1661-6596en
dc.identifier.urihttps://hdl.handle.net/10356/80456-
dc.description.abstractBone transplants are used to treat fractures and increase new tissue development in bone tissue engineering. Grafting of massive implantations showing slow curing rate and results in cell death for poor vascularization. The potentials of biocomposite scaffolds to mimic extracellular matrix (ECM) and including new biomaterials could produce a better substitute for new bone tissue formation. A purpose of this study is to analyze polycaprolactone/silk fibroin/hyaluronic acid/minocycline hydrochloride (PCL/SF/HA/MH) nanoparticles initiate human mesenchymal stem cells (MSCs) proliferation and differentiation into osteogenesis. Electrospraying technique was used to develop PCL, PCL/SF, PCL/SF/HA and PCL/SF/HA/MH hybrid biocomposite nanoparticles and characterization was analyzed by field emission scanning electron microscope (FESEM), contact angle and Fourier transform infrared spectroscopy (FT-IR). The obtained results proved that the particle diameter and water contact angle obtained around 0.54 ± 0.12 to 3.2 ± 0.18 µm and 43.93 ± 10.8° to 133.1 ± 12.4° respectively. The cell proliferation and cell-nanoparticle interactions analyzed using (3-(4,5-dimethyl thiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium inner salt) MTS assay (Promega, Madison, WI, USA), FESEM for cell morphology and 5-Chloromethylfluorescein diacetate (CMFDA) dye for imaging live cells. Osteogenic differentiation was proved by expression of osteocalcin, alkaline phosphatase activity (ALP) and mineralization was confirmed by using alizarin red (ARS). The quantity of cells was considerably increased in PCL/SF/HA/MH nanoparticles when compare to all other biocomposite nanoparticles and the cell interaction was observed more on PCL/SF/HA/MH nanoparticles. The electrosprayed PCL/SF/HA/MH biocomposite nanoparticle significantly initiated increased cell proliferation, osteogenic differentiation and mineralization, which provide huge potential for bone tissue engineering.en
dc.description.sponsorshipMOE (Min. of Education, S’pore)en
dc.format.extent16 p.en
dc.language.isoenen
dc.relation.ispartofseriesInternational Journal of Molecular Sciencesen
dc.rights© 2016 by The Author(s); licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).en
dc.subjectElectrosprayingen
dc.subjectPolycaprolactoneen
dc.subjectDRNTU::Science::Medicineen
dc.titleMinocycline loaded hybrid composites nanoparticles for mesenchymal stem cells differentiation into osteogenesisen
dc.typeJournal Articleen
dc.contributor.schoolLee Kong Chian School of Medicine (LKCMedicine)en
dc.contributor.organizationCellular and Molecular Epigenetics Laben
dc.identifier.doi10.3390/ijms17081222en
dc.description.versionPublished versionen
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Appears in Collections:LKCMedicine Journal Articles
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