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Title: Control of plasma membrane lipid homeostasis by the extended synaptotagmins
Authors: Saheki, Yasunori
Bian, Xin
Schauder, Curtis M.
Sawaki, Yujin
Surma, Michal A.
Klose, Christian
Pincet, Frederic
Reinisch, Karin M.
De Camilli, Pietro
Keywords: Calcium signalling
Endoplasmic reticulum
Lipid signalling
Membrane trafficking
Issue Date: 2016
Source: Saheki, Y., Bian, X., Schauder, C. M., Sawaki, Y., Surma, M. A., Klose, C., et al. (2016). Control of plasma membrane lipid homeostasis by the extended synaptotagmins. Nature Cell Biology, 18(5), 504-515.
Series/Report no.: Nature Cell Biology
Abstract: Acute metabolic changes in plasma membrane (PM) lipids, such as those mediating signalling reactions, are rapidly compensated by homeostatic responses whose molecular basis is poorly understood. Here we show that the extended synaptotagmins (E-Syts), endoplasmic reticulum (ER) proteins that function as PtdIns(4,5)P2- and Ca2+-regulated tethers to the PM, participate in these responses. E-Syts transfer glycerolipids between bilayers in vitro, and this transfer requires Ca2+ and their lipid-harbouring SMP domain. Genome-edited cells lacking E-Syts do not exhibit abnormalities in the major glycerolipids at rest, but exhibit enhanced and sustained accumulation of PM diacylglycerol following PtdIns(4,5)P2 hydrolysis by PLC activation, which can be rescued by expression of E-Syt1, but not by mutant E-Syt1 lacking the SMP domain. The formation of E-Syt-dependent ER–PM tethers in response to stimuli that cleave PtdIns(4,5)P2 and elevate Ca2+ may help reverse accumulation of diacylglycerol in the PM by transferring it to the ER for metabolic recycling.
ISSN: 1465-7392
DOI: 10.1038/ncb3339
Schools: Lee Kong Chian School of Medicine (LKCMedicine) 
Rights: © 2016 Macmillan Publishers Ltd. This is the author created version of a work that has been peer reviewed and accepted for publication by Nature Cell Biology, Macmillan Publishers Ltd. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [].
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:LKCMedicine Journal Articles

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