Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/80636
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dc.contributor.authorJayaraman, Praveenaen
dc.contributor.authorGandhimathi, Chinnasamyen
dc.contributor.authorVenugopal, Jayarama Reddyen
dc.contributor.authorRamakrishna, Seeramen
dc.contributor.authorSrinivasan, Dinesh Kumaren
dc.date.accessioned2016-06-06T07:51:28Zen
dc.date.accessioned2019-12-06T13:53:37Z-
dc.date.available2016-06-06T07:51:28Zen
dc.date.available2019-12-06T13:53:37Z-
dc.date.copyright2016en
dc.date.issued2016en
dc.identifier.citationJayaraman, P., Gandhimathi, C., Venugopal, J. R., Ramakrishna, S., & Srinivasan, D. K. (2016). Minocycline hydrochloride entrapped biomimetic nanofibrous substitutes for adipose derived stem cells differentiation into osteogenesis. Regenerative Engineering and Translational Medicine, 2(1), 10-22.en
dc.identifier.issn2364-4133en
dc.identifier.urihttps://hdl.handle.net/10356/80636-
dc.description.abstractHybrid biocomposite nanofibrous structures that mimics native extracellular matrix have been extensively applied for bone tissue engineering (BTE) due to their potential in efficiently inducing cellular response for the secretion of extracellular matrix (ECM). This study performed fabrication of uniform porous polycaprolactone (PCL), polycaprolactone/silk fibroin (PCL/SF), polycaprolactone/silk fibroin/minocycline hydrochloride (PCL/SF/MH), polycaprolactone/collagen (PCL/COL), and polycaprolactone/collagen/minocycline hydrochloride (PCL/COL/MH) biocomposites nanofibrous scaffolds by electrospinning, for comparing their properties to use in bone tissue regeneration. Field emission scanning electron microscopy (FESEM) images of fabricated nanofibrous scaffolds revealed porous, beadless, uniform fibers of diameter in the range of 147.13 ± 28.02 to 176.53 ± 22.34 nm and porosity around 82–93 %. Adipose-derived stem cells (ADSCs) considered as the novel cell therapeutics were cultured on these electrospun fibrous scaffolds to undergo osteogenic differentiation for BTE. The cell morphology, proliferation, and interactions were analyzed by CMFDA dye extrusion, MTS assay, and FESEM analysis, respectively. Differentiation of ADSCs into osteogenesis was determined by alkaline phosphatase activity, mineralization by alizarin red staining, and osteogenic protein expression by immunofluorescence analysis. The results demonstrated that the addition of SF and MH to PCL-based scaffolds improved the mechanical stability, interconnected pores, and surface roughness of the scaffolds initiating heightened biological functions such as ADSCs adhesion, proliferation, differentiation, and mineralization into osteogenesis for bone tissue regeneration.en
dc.description.sponsorshipMOE (Min. of Education, S’pore)en
dc.language.isoenen
dc.relation.ispartofseriesRegenerative Engineering and Translational Medicineen
dc.rights© 2016 The Regenerative Engineering Society.en
dc.subjectPolycaprolactoneen
dc.subjectSilk fibroinen
dc.subjectCollagenen
dc.subjectMinocycline hydrochlorideen
dc.subjectMineralizationen
dc.subjectDifferentiationen
dc.subjectBone tissue engineeringen
dc.titleMinocycline hydrochloride entrapped biomimetic nanofibrous substitutes for adipose derived stem cells differentiation into osteogenesisen
dc.typeJournal Articleen
dc.contributor.schoolLee Kong Chian School of Medicine (LKCMedicine)en
dc.identifier.doi10.1007/s40883-016-0010-yen
dc.identifier.rims189296en
item.fulltextNo Fulltext-
item.grantfulltextnone-
Appears in Collections:LKCMedicine Journal Articles

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