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Title: Pronounced peptide selectivity for melanoma through tryptophan end-tagging
Authors: Duong, Dinh Thuy
Singh, Shalini
Bagheri, Mojtaba
Verma, Navin Kumar
Schmidtchen, Artur
Malmsten, Martin
Issue Date: 2016
Source: Duong, D. T., Singh, S., Bagheri, M., Verma, N. K., Schmidtchen, A., & Malmsten, M. (2016). Pronounced peptide selectivity for melanoma through tryptophan end-tagging. Scientific Reports, 6, 24952-.
Series/Report no.: Scientific Reports
Abstract: Effects of oligotryptophan end-tagging on the uptake of arginine-rich peptides into melanoma cells was investigated under various conditions and compared to that into non-malignant keratinocytes, fibroblasts, biophysical studies on peptide binding to, and destabilization of, model lipid membranes provided mechanistic insight into the origin of the selectivity between melanoma and non-malignant cells. Collectively, the results demonstrate that W-tagging represents a powerful way to increase selective peptide internalization in melanoma cells, resulting in toxicity against these, but not against the non-malignant cells. These and erythrocytes, also monitoring resulting cell toxicity. In parallel, effects were shown to be due to increased peptide adsorption to the outer membrane in melanoma cells, caused by the presence of anionic lipids such as phosphatidylserine and ganglioside GM1, and to peptide effects on mitochondria membranes and resulting apoptosis. In addition, the possibility of using W-tagged peptides for targeted uptake of nanoparticles/drug carriers in melanoma was demonstrated, as was the possibility to open up the outer membrane of melanoma cells in order to facilitate uptake of low Mw anticancer drugs, here demonstrated for dox0rubicin.
ISSN: 2045-2322
DOI: 10.1038/srep24952
Schools: Lee Kong Chian School of Medicine (LKCMedicine) 
Rights: © 2016 The Authors. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:LKCMedicine Journal Articles

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