Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/80710
Title: Cell-selective pore forming antimicrobial peptides of the prodomain of human furin : a conserved aromatic/cationic sequence mapping, membrane disruption, and atomic-resolution structure and dynamics
Authors: Sinha, Sheetal
Harioudh, Munesh Kumar
Dewangan, Rikeshwer P.
Ng, Wun Jern
Ghosh, Jimut Kanti
Bhattacharjya, Surajit
Keywords: Pore Forming
Human Furin
Science::Biological sciences
Issue Date: 2018
Source: Sinha, S., Harioudh, M. K., Dewangan, R. P., Ng, W. J., Ghosh, J. K., & Bhattacharjya, S. (2018). Cell-selective pore forming antimicrobial peptides of the prodomain of human furin : a conserved aromatic/cationic sequence mapping, membrane disruption, and atomic-resolution structure and dynamics. ACS Omega, 3(11), 14650-14664. doi:10.1021/acsomega.8b01876
Series/Report no.: ACS Omega
Abstract: Antimicrobial peptides are promising molecules in uprising consequences of drug-resistant bacteria. The prodomain of furin, a serine protease, expressed in all vertebrates including humans, is known to be important for physiological functions. Here, potent antimicrobial peptides were mapped by extensive analyses of overlapping peptide fragments of the prodomain of human furin. Two peptides, YR26 and YR23, were active against bacterial cells including MRSA-resistant Staphylococcus aureus and Staphylococcus epidermis 51625. Peptides were largely devoid of hemolytic and cytotoxic activity. Bacterial cell killing occurred as a result of the disruption of the permeability barrier of the lipopolysaccharide (LPS)-outer membrane and fragmentation of LPS into small micelles. Furthermore, antibacterial peptides specifically interacted with the negatively charged lipids causing membrane leakage and fusion. The YR26 peptide in sodium dodecyl sulfate micelles demonstrated a long-helix-turn-short-helix structure exhibiting restricted backbone motions. The cell-selective activity of the furin peptides and their unique mode of action on membranes have a significant potential for the development of therapeutics.
URI: https://hdl.handle.net/10356/80710
http://hdl.handle.net/10220/50373
DOI: 10.1021/acsomega.8b01876
Rights: © 2018 American Chemical Society. This is an open access article published under an ACS AuthorChoice License, which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SBS Journal Articles

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