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Title: Decellularization of human stromal refractive lenticules for corneal tissue engineering
Authors: Yam, Gary Hin-Fai
Yusoff, Nur Zahirah Binte M.
Goh, Tze-Wei
Setiawan, Melina
Lee, Xiao-Wen
Liu, Yu-Chi
Mehta, Jodhbir Singh
Keywords: Tissue Engineering
Corneal Stroma
Issue Date: 2016
Source: Yam, G. H.-F., Yusoff, N. Z. B. M., Goh, T.-W., Setiawan, M., Lee, X.-W., Liu, Y.-C., & Mehta, J. S. (2016). Decellularization of human stromal refractive lenticules for corneal tissue engineering. Scientific Reports, 6, 26339-. doi:10.1038/srep26339
Series/Report no.: Scientific Reports
Abstract: Small incision lenticule extraction (SMILE) becomes a procedure to correct myopia. The extracted lenticule can be used for other clinical scenarios. To prepare for allogeneic implantation, lenticule decellularization with preserved optical property, stromal architecture and chemistry would be necessary. We evaluated different methods to decellularize thin human corneal stromal lenticules created by femtosecond laser. Treatment with 0.1% sodium dodecylsulfate (SDS) followed by extensive washes was the most efficient protocol to remove cellular and nuclear materials. Empty cell space was found inside the stroma, which displayed aligned collagen fibril architecture similar to native stroma. The SDS-based method was superior to other treatments with hyperosmotic 1.5 M sodium chloride, 0.1% Triton X-100 and nucleases (from 2 to 10 U/ml DNase and RNase) in preserving extracellular matrix content (collagens, glycoproteins and glycosaminoglycans). The stromal transparency and light transmittance was indifferent to untreated lenticules. In vitro recellularization showed that the SDS-treated lenticules supported corneal stromal fibroblast growth. In vivo re-implantation into a rabbit stromal pocket further revealed the safety and biocompatibility of SDS-decellularized lenticules without short- and long-term rejection risk. Our results concluded that femtosecond laser-derived human stromal lenticules decellularized by 0.1% SDS could generate a transplantable bioscaffold with native-like stromal architecture and chemistry.
DOI: 10.1038/srep26339
Schools: School of Materials Science & Engineering 
Rights: © 2016 The Authors (Nature Publishing Group). This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:MSE Journal Articles

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