Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/80921
Title: Caveolae provide a specialized membrane environment for respiratory syncytial virus assembly
Authors: Ravi, Laxmi Iyer
Huan, Tan Boon
Ludwig, Alexander
Nguyen, Tra Huong
Leong, Daniel
Sandin, Sara
Sugrue, Richard J.
Keywords: Caveolin
Caveolae
Issue Date: 2017
Source: Ludwig, A., Nguyen, T. H., Leong, D., Ravi, L. I., Huan, T. B., Sandin, S., et al. (2017). Caveolae provide a specialized membrane environment for respiratory syncytial virus assembly. Journal of Cell Science, 130, 1037-1050.
Series/Report no.: Journal of Cell Science
Abstract: Respiratory syncytial virus (RSV) is an enveloped virus that assembles into filamentous virus particles on the surface of infected cells. Morphogenesis of RSV is dependent upon cholesterol-rich (lipid raft) membrane microdomains, but the specific role of individual raft molecules in RSV assembly is not well defined. Here, we show that RSV morphogenesis occurs within caveolar membranes and that both caveolin-1 and cavin-1 (also known as PTRF), the two major structural and functional components of caveolae, are actively recruited to and incorporated into the RSV envelope. The recruitment of caveolae occurred just prior to the initiation of RSV filament assembly, and was dependent upon an intact actin network as well as a direct physical interaction between caveolin-1 and the viral G protein. Moreover, cavin-1 protein levels were significantly increased in RSV-infected cells, leading to a virus-induced change in the stoichiometry and biophysical properties of the caveolar coat complex. Our data indicate that RSV exploits caveolae for its assembly, and we propose that the incorporation of caveolae into the virus contributes to defining the biological properties of the RSV envelope.
URI: https://hdl.handle.net/10356/80921
http://hdl.handle.net/10220/43442
ISSN: 0021-9533
DOI: 10.1242/jcs.198853
Schools: School of Biological Sciences 
Rights: © 2017 The Author(s) (published by The Company of Biologists Ltd). This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SBS Journal Articles

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