Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/80936
Title: A newly discovered Bordetella species carries a transcriptionally active CRISPR-Cas with a small Cas9 endonuclease
Authors: Ivanov, Yury V.
Shariat, Nikki
Register, Karen B.
Linz, Bodo
Rivera, Israel
Hu, Kai
Dudley, Edward G.
Harvill, Eric Thomas
Keywords: Bordetella pseudohinzii
Bacteria
Genome editing
Protospacer
Type II CRISPR
Cas9
SpyCas9
GC-content
HGT
Issue Date: 2015
Source: Ivanov, Y. V., Shariat, N., Register, K. B., Linz, B., Rivera, I., Hu, K., et al (2015). A newly discovered Bordetella species carries a transcriptionally active CRISPR-Cas with a small Cas9 endonuclease. BMC Genomics, 16(863).
Series/Report no.: BMC Genomics
Abstract: Background: Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated genes (cas) are widely distributed among bacteria. These systems provide adaptive immunity against mobile genetic elements specified by the spacer sequences stored within the CRISPR. Methods: The CRISPR-Cas system has been identified using Basic Local Alignment Search Tool (BLAST) against other sequenced and annotated genomes and confirmed via CRISPRfinder program. Using Polymerase Chain Reactions (PCR) and Sanger DNA sequencing, we discovered CRISPRs in additional bacterial isolates of the same species of Bordetella. Transcriptional activity and processing of the CRISPR have been assessed via RT-PCR. Results: Here we describe a novel Type II-C CRISPR and its associated genes—cas1, cas2, and cas9—in several isolates of a newly discovered Bordetella species. The CRISPR-cas locus, which is absent in all other Bordetella species, has a significantly lower GC-content than the genome-wide average, suggesting acquisition of this locus via horizontal gene transfer from a currently unknown source. The CRISPR array is transcribed and processed into mature CRISPR RNAs (crRNA), some of which have homology to prophages found in closely related species B. hinzii. Conclusions: Expression of the CRISPR-Cas system and processing of crRNAs with perfect homology to prophages present in closely related species, but absent in that containing this CRISPR-Cas system, suggest it provides protection against phage predation. The 3,117-bp cas9 endonuclease gene from this novel CRISPR-Cas system is 990 bp smaller than that of Streptococcus pyogenes, the 4,017-bp allele currently used for genome editing, and which may make it a useful tool in various CRISPR-Cas technologies.
URI: https://hdl.handle.net/10356/80936
http://hdl.handle.net/10220/38980
ISSN: 1471-2164
DOI: 10.1186/s12864-015-2028-9
Rights: © 2015 Ivanov et al. Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:LKCMedicine Journal Articles

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