Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/80938
Title: Dengue Serotype Cross-Reactive, Anti-E Protein Antibodies Confound Specific Immune Memory for 1 Year after Infection
Authors: Toh, Ying Xiu
Gan, Victor
Balakrishnan, Thavamalar
Zuest, Roland
Poidinger, Michael
Wilson, Solomonraj
Appanna, Ramapraba
Thein, Tun Linn
Ong, Adrian Kheng-Yeow
Ng, Lee Ching
Leo, Yee Sin
Fink, Katja
Keywords: B cells
Dengue
Viral infection
Antibodies
Plasmablasts
Longitudinal studies
Cross-reactive
Vaccines
Issue Date: 2014
Source: Toh, Y. X., Gan, V., Balakrishnan, T., Zuest, R., Poidinger, M., Wilson, S., et al. (2014). Dengue Serotype Cross-Reactive, Anti-E Protein Antibodies Confound Specific Immune Memory for 1 Year after Infection. Frontiers in Immunology, 5(388).
Series/Report no.: Frontiers in Immunology
Abstract: Dengue virus has four serotypes and is endemic globally in tropical countries. Neither a specific treatment nor an approved vaccine is available, and correlates of protection are not established. The standard neutralization assay cannot differentiate between serotype-specific and serotype cross-reactive antibodies in patients early after infection, leading to an overestimation of the long-term serotype-specific protection of an antibody response. It is known that the cross-reactive response in patients is temporary but few studies have assessed kinetics and potential changes in serum antibody specificity over time. To better define the specificity of polyclonal antibodies during disease and after recovery, longitudinal samples from patients with primary or secondary DENV-2 infection were collected over a period of 1 year. We found that serotype cross-reactive antibodies peaked 3 weeks after infection and subsided within 1 year. Since secondary patients rapidly produced antibodies specific for the virus envelope (E) protein, an E-specific ELISA was superior compared to a virus particle-specific ELISA to identify patients with secondary infections. Dengue infection triggered a massive activation and mobilization of both naïve and memory B cells possibly from lymphoid organs into the blood, providing an explanation for the surge of circulating plasmablasts and the increase in cross-reactive E protein-specific antibodies.
URI: https://hdl.handle.net/10356/80938
http://hdl.handle.net/10220/38982
ISSN: 1664-3224
DOI: 10.3389/fimmu.2014.00388
Rights: © 2014 Toh, Gan, Balakrishnan, Zuest, Poidinger, Wilson, Appanna, Thein, Ong, Ng, Leo and Fink. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:LKCMedicine Journal Articles

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