Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/80945
Title: Proteomics analysis of metabolically engineered yeast cells and medium-chained hydrocarbon biofuel precursors synthesis
Authors: Li, Xiang
Chen, Wei Ning
Keywords: Hydroperoxide lyase
Saccharomyces cerevisiae
Lipoxygenase
Medium-chained biofuel precursors
Proteomics
Issue Date: 2014
Source: Li, X., & Chen, W. N. (2014). Proteomics analysis of metabolically engineered yeast cells and medium-chained hydrocarbon biofuel precursors synthesis. AMB Express, 4, 61-.
Series/Report no.: AMB Express
Abstract: Recently, various biofuels have been synthesized through metabolic engineering approaches to meet the exploding energy demands. Hydrocarbon biofuels, energy-equivalent to petroleum-based fuels, are identified as promising replacements for petroleum. Metabolically engineered Saccharomyces cerevisiae capable of synthesize precursors of medium-chained hydrocarbons is proposed in this study. The hydroperoxide pathway introduced in S. cerevisiae consisted of lipoxygenase (LOX) and hydroperoxide lyase (HPL) from almond, which catalyzes linoleic acid to 3(Z)-nonenal, the precursor for medium-chained hydrocarbon biofuels. Proteomics study showed that 31 proteins displayed different expression levels among four functional strains and most of them were related to carbohydrate metabolism and protein synthesis, suggested prospective capabilities of energy generation and exogenous protein synthesis. Biotransformation efficiency studies carried out by GC-FID were in accordance with the expectations. The highest yield of 3(Z)-nonenal was up to 1.21 ± 0.05 mg/L with the carbon recovery of up to 12.4%.
URI: https://hdl.handle.net/10356/80945
http://hdl.handle.net/10220/39031
ISSN: 2191-0855
DOI: 10.1186/s13568-014-0061-8
Schools: School of Chemical and Biomedical Engineering 
Rights: © 2014 Li and Chen; licensee Springer. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SCBE Journal Articles

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