Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/80952
Title: Survival and persistence of host-associated Bacteroidales cells and DNA in comparison with Escherichia coli and Enterococcus in freshwater sediments as quantified by PMA-qPCR and qPCR
Authors: Kim, Minji
Wuertz, Stefan
Keywords: Bacteroidales
Fecal indicator bacteria
Propidium monoazide
Sediment
Microbial source tracking
Quantitative PCR
Issue Date: 2015
Source: Kim, M., & Wuertz, S. (2015). Survival and persistence of host-associated Bacteroidales cells and DNA in comparison with Escherichia coli and Enterococcus in freshwater sediments as quantified by PMA-qPCR and qPCR. Water Research, 87, 182-192.
Series/Report no.: Water Research
Abstract: Decay of the fecal source identifier Bacteroidales in sediments has not been studied until now. Two types of microcosms inoculated with human, cow and dog feces were constructed to investigate the survival and persistence of host-associated Bacteroidales cells and their DNA, respectively, in freshwater sediments: (i) a completely anaerobic microcosm where feces were entirely mixed with sediments for estimating decay of Bacteroidales in oxygen-free sediments at two temperatures (6 °C and 20 °C) and (ii) a core microcosm where feces in the overlying water column settled on top of undisturbed core sediments. Quantitative PCR (qPCR) along with propidium monoazide (PMA) was used to differentiate between genetic markers present in intact cells and total intracellular as well as extracellular marker DNA. Regulated fecal indicator bacteria were measured by cultivation (Escherichia coli and Enterococcus) and qPCR (Enterococcus) in relation to Bacteroidales-associated host markers. In anaerobic microcosms, the survival and persistence of Bacteroidales cells and DNA in sediments were considerably extended, especially at the lower temperature of 6 °C, with two-log reduction times (T99) >56 d (cells) and >169 d (DNA). Bacteroidales DNA persisted up to five times longer than cells in anaerobic microcosms at 6 °C, whereas decay rates of cells and DNA were not significantly different at 20 °C in anaerobic microcosms. In core microcosms, the levels of Bacteroidales cells and DNA decreased approximately six times more slowly in sediments than in overlying water; T99 values of Bacteroidales cells and DNA were 6–9 d (water) and 29–82 d (sediment). The survival of universal, human-, ruminant- and dog-associated Bacteroidales cells in sediments was similar in both microcosms under each given condition, as was the persistence of DNA. Decay rate constants of Bacteroidales cells and DNA were comparable with those of cultivable Enterococcus and E. coli cells in core sediments while Enterococcus DNA levels fluctuated without noticeable decay. The prolonged persistence of host-associated Bacteroidales suggests that sediments should be considered in practical applications of microbial source tracking, because they can act as non-point sources of fecal markers.
URI: https://hdl.handle.net/10356/80952
http://hdl.handle.net/10220/38977
ISSN: 0043-1354
DOI: 10.1016/j.watres.2015.09.014
Schools: School of Civil and Environmental Engineering 
Research Centres: Singapore Centre for Environmental Life Sciences Engineering 
Rights: © 2015 Elsevier Ltd. This is the author created version of a work that has been peer reviewed and accepted for publication by Water Research, Elsevier Ltd. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1016/j.watres.2015.09.014].
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:CEE Journal Articles
SCELSE Journal Articles

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