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Title: Microsphere integrated microfluidic disk: synergy of two techniques for rapid and ultrasensitive dengue detection
Authors: Hosseini, Samira
Aeinehvand, Mohammad M.
Uddin, Shah M.
Benzina, Abderazak
Rothan, Hussin A.
Yusof, Rohana
Koole, Leo H.
Madou, Marc J.
Djordjevic, Ivan
Ibrahim, Fatimah
Issue Date: 2015
Source: Hosseini, S., Aeinehvand, M. M., Uddin, S. M., Benzina, A., Rothan, H. A., Yusof, R., et al. Microsphere integrated microfluidic disk: synergy of two techniques for rapid and ultrasensitive dengue detection. Scientific Reports, 5, 16485-.
Series/Report no.: Scientific Reports
Abstract: The application of microfluidic devices in diagnostic systems is well-established in contemporary research. Large specific surface area of microspheres, on the other hand, has secured an important position for their use in bioanalytical assays. Herein, we report a combination of microspheres and microfluidic disk in a unique hybrid platform for highly sensitive and selective detection of dengue virus. Surface engineered polymethacrylate microspheres with carefully designed functional groups facilitate biorecognition in a multitude manner. In order to maximize the utility of the microspheres’ specific surface area in biomolecular interaction, the microfluidic disk was equipped with a micromixing system. The mixing mechanism (microballoon mixing) enhances the number of molecular encounters between spheres and target analyte by accessing the entire sample volume more effectively, which subsequently results in signal amplification. Significant reduction of incubation time along with considerable lower detection limits were the prime motivations for the integration of microspheres inside the microfluidic disk. Lengthy incubations of routine analytical assays were reduced from 2 hours to 5 minutes while developed system successfully detected a few units of dengue virus. Obtained results make this hybrid microsphere-microfluidic approach to dengue detection a promising avenue for early detection of this fatal illness.
ISSN: 2045-2322
DOI: 10.1038/srep16485
Schools: School of Materials Science & Engineering 
Rights: This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit
Fulltext Permission: open
Fulltext Availability: With Fulltext
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