Please use this identifier to cite or link to this item:
Full metadata record
DC FieldValueLanguage
dc.contributor.authorMundargi, Raghavendra C.en
dc.contributor.authorPotroz, Michael G.en
dc.contributor.authorPark, Jae Hyeonen
dc.contributor.authorSeo, Jeongeunen
dc.contributor.authorTan, Ee-Linen
dc.contributor.authorLee, Jae Hoen
dc.contributor.authorCho, Nam-Joonen
dc.identifier.citationMundargi, R. C., Potroz, M. G., Park, J. H., Seo, J., Tan, E.-L., Lee, J. H., & Cho, N.-J. (2016). Eco-friendly streamlined process for sporopollenin exine capsule extraction. Scientific Reports, 6, 19960-. doi:10.1038/srep19960en
dc.description.abstractSporopollenin exine capsules (SECs) extracted from Lycopodium clavatum spores are an attractive biomaterial possessing a highly robust structure suitable for microencapsulation strategies. Despite several decades of research into SEC extraction methods, the protocols commonly used for L. clavatum still entail processing with both alkaline and acidolysis steps at temperatures up to 180 °C and lasting up to 7 days. Herein, we demonstrate a significantly streamlined processing regimen, which indicates that much lower temperatures and processing durations can be used without alkaline lysis. By employing CHN elemental analysis, scanning electron microscopy (SEM), confocal laser scanning microscopy (CLSM), and dynamic image particle analysis (DIPA), the optimum conditions for L. clavatum SEC processing were determined to include 30 hours acidolysis at 70 °C without alkaline lysis. Extending these findings to proof-of-concept encapsulation studies, we further demonstrate that our SECs are able to achieve a loading of 0.170 ± 0.01 g BSA per 1 g SECs by vacuum-assisted loading. Taken together, our streamlined processing method and corresponding characterization of SECs provides important insights for the development of applications including drug delivery, cosmetics, personal care products, and foods.en
dc.description.sponsorshipNRF (Natl Research Foundation, S’pore)en
dc.description.sponsorshipNMRC (Natl Medical Research Council, S’pore)en
dc.format.extent14 p.en
dc.relation.ispartofseriesScientific Reportsen
dc.rights© 2016 The Authors (Nature Publishing Group). This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit
dc.subjectPlant Extractsen
dc.titleEco-friendly streamlined process for sporopollenin exine capsule extractionen
dc.typeJournal Articleen
dc.contributor.schoolSchool of Chemical and Biomedical Engineeringen
dc.contributor.schoolSchool of Materials Science & Engineeringen
dc.contributor.researchCentre for Biomimetic Sensor Scienceen
dc.description.versionPublished versionen
item.fulltextWith Fulltext-
Appears in Collections:MSE Journal Articles
SCBE Journal Articles
Files in This Item:
File Description SizeFormat 
Eco-friendly streamlined process for sporopollenin exine capsule extraction.pdf3.56 MBAdobe PDFThumbnail

Citations 5

Updated on Feb 15, 2022

Citations 5

Updated on Feb 8, 2022

Page view(s)

Updated on May 21, 2022

Download(s) 50

Updated on May 21, 2022

Google ScholarTM




Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.