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Title: Real-time and non-invasive monitoring of embryonic stem cell survival during the development of embryoid bodies with smart nanosensor
Authors: Fu, Jiayin
Wiraja, Christian
Chong, Ruiqi
Xu, Chenjie
Wang, Dong-An
Keywords: Embryonic stem cell
Embryoid body
Issue Date: 2016
Source: Fu, J., Wiraja, C., Chong, R., Xu, C., & Wang, D.-A. (2017). Real-time and non-invasive monitoring of embryonic stem cell survival during the development of embryoid bodies with smart nanosensor. Acta Biomaterialia, 49, 358-367.
Series/Report no.: Acta Biomaterialia
Abstract: Embryonic stem cells (ESCs)-derived embryoid body (EB) is a powerful model for the study of early embryonic development and the discovery of therapeutics for tissue regeneration. This article reports a smart nanosensor platform for labeling and tracking the survival and distribution of ESCs during the EB development in a real-time and non-invasive way. Compared with the cell tracker (i.e. DiO) and the green fluorescent protein (GFP), nanosensors provide the homogenous and highly-efficient ESC labeling. Following the internalization, intracellular nanosensors gradually release the non-fluorescent molecules that become fluorescent only in viable cells. This allows a continuous monitoring of ESC survival and distribution during the process of EB formation. Finally, we confirm that nanosensor labeling does not cause the significant influences to biological properties of the ESCs and EBs.
ISSN: 1742-7061
DOI: 10.1016/j.actbio.2016.11.027
Rights: © 2016 Acta Materialia Inc. (published by Elsevier Ltd.). This is the author created version of a work that has been peer reviewed and accepted for publication in Acta Biomaterialia, published by Elsevier Ltd. on behalf of Acta Materialia Inc. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document.  The published version is available at: [].
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SCBE Journal Articles

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