Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/81057
Full metadata record
DC FieldValueLanguage
dc.contributor.authorBhave, Madhura Satishen
dc.contributor.authorHassanbhai, Ammar Mansooren
dc.contributor.authorAnand, Padmajaen
dc.contributor.authorLuo, Kathy Qianen
dc.contributor.authorTeoh, Swee Hinen
dc.date.accessioned2015-12-14T01:18:28Zen
dc.date.accessioned2019-12-06T14:20:31Z-
dc.date.available2015-12-14T01:18:28Zen
dc.date.available2019-12-06T14:20:31Z-
dc.date.issued2015en
dc.identifier.citationBhave, M. S., Hassanbhai, A. M., Anand, P., Luo, K. Q., & Teoh, S. H. (2015). Effect of Heat-Inactivated Clostridium sporogenes and Its Conditioned Media on 3-Dimensional Colorectal Cancer Cell Models. Scientific Reports, 5, 15681-.en
dc.identifier.issn2045-2322en
dc.identifier.urihttps://hdl.handle.net/10356/81057-
dc.description.abstractTraditional cancer treatments, such as chemotherapy and radiation therapy continue to have limited efficacy due to tumor hypoxia. While bacterial cancer therapy has the potential to overcome this problem, it comes with the risk of toxicity and infection. To circumvent these issues, this paper investigates the anti-tumor effects of non-viable bacterial derivatives of Clostridium sporogenes. These non-viable derivatives are heat-inactivated C. sporogenes bacteria (IB) and the secreted bacterial proteins in culture media, known as conditioned media (CM). In this project, the effects of IB and CM on CT26 and HCT116 colorectal cancer cells were examined on a 2-Dimensional (2D) and 3-Dimensional (3D) platform. IB significantly inhibited cell proliferation of CT26 to 6.3% of the control in 72 hours for the 2D monolayer culture. In the 3D spheroid culture, cell proliferation of HCT116 spheroids notably dropped to 26.2%. Similarly the CM also remarkably reduced the cell-proliferation of the CT26 cells to 2.4% and 20% in the 2D and 3D models, respectively. Interestingly the effect of boiled conditioned media (BCM) on the cells in the 3D model was less inhibitory than that of CM. Thus, the inhibitive effect of inactivated C. sporogenes and its conditioned media on colorectal cancer cells is established.en
dc.format.extent12 p.en
dc.language.isoenen
dc.relation.ispartofseriesScientific Reportsen
dc.rightsThis work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/en
dc.titleEffect of Heat-Inactivated Clostridium sporogenes and Its Conditioned Media on 3-Dimensional Colorectal Cancer Cell Modelsen
dc.typeJournal Articleen
dc.contributor.schoolSchool of Chemical and Biomedical Engineeringen
dc.identifier.doi10.1038/srep15681en
dc.description.versionPublished versionen
dc.identifier.pmid26507312-
item.fulltextWith Fulltext-
item.grantfulltextopen-
Appears in Collections:SCBE Journal Articles

SCOPUSTM   
Citations 20

21
Updated on Jun 17, 2024

Web of ScienceTM
Citations 20

12
Updated on Oct 29, 2023

Page view(s) 50

580
Updated on Jun 24, 2024

Download(s) 20

196
Updated on Jun 24, 2024

Google ScholarTM

Check

Altmetric


Plumx

Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.