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Title: Smooth Muscle Cell Alignment and Phenotype Control by Melt Spun Polycaprolactone Fibers for Seeding of Tissue Engineered Blood Vessels
Authors: Agrawal, Animesh
Lee, Bae Hoon
Irvine, Scott Alexander
An, Jia
Bhuthalingam, Ramya
Singh, Vaishali
Low, Kok Yao
Chua, Chee Kai
Venkatraman, Subbu Subramanian
Issue Date: 2015
Source: Agrawal, A., Lee, B. H., Irvine, S. A., An, J., Bhuthalingam, R., Singh, V., et al. (2015). Smooth Muscle Cell Alignment and Phenotype Control by Melt Spun Polycaprolactone Fibers for Seeding of Tissue Engineered Blood Vessels. International Journal of Biomaterials, 2015, 434876-.
Series/Report no.: International Journal of Biomaterials
Abstract: A method has been developed to induce and retain a contractile phenotype for vascular smooth muscle cells, as the first step towards the development of a biomimetic blood vessel construct with minimal compliance mismatch. Melt spun PCL fibers were deposited on a mandrel to form aligned fibers of 10 μm in diameter. The fibers were bonded into aligned arrangement through dip coating in chitosan solution. This formed a surface of parallel grooves, 10 μm deep by 10 μm across, presenting a surface layer of chitosan to promote cell surface interactions. The aligned fiber surface was used to culture cells present in the vascular wall, in particular fibroblasts and smooth muscle cells. This topography induced “surface guidance” over the orientation of the cells, which adopted an elongated spindle-like morphology, whereas cells on the unpatterned control surface did not show such orientation, assuming more rhomboid shapes. The preservation of VSMC contractile phenotype on the aligned scaffold was demonstrated by the retention of α-SMA expression after several days of culture. The effect was assessed on a prototype vascular graft prosthesis fabricated from polylactide caprolactone; VSMCs aligned longitudinally along a fiberless tube, whereas, for the aligned fiber coated tubes, the VSMCs aligned in the required circumferential orientation.
ISSN: 1687-8787
DOI: 10.1155/2015/434876
Schools: School of Materials Science & Engineering 
School of Mechanical and Aerospace Engineering 
School of Biological Sciences 
Rights: © 2015 Animesh Agrawal et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Fulltext Permission: open
Fulltext Availability: With Fulltext
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