Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/81091
Title: Smooth Muscle Cell Alignment and Phenotype Control by Melt Spun Polycaprolactone Fibers for Seeding of Tissue Engineered Blood Vessels
Authors: Agrawal, Animesh
Lee, Bae Hoon
Irvine, Scott Alexander
An, Jia
Bhuthalingam, Ramya
Singh, Vaishali
Low, Kok Yao
Chua, Chee Kai
Venkatraman, Subbu Subramanian
Issue Date: 2015
Source: Agrawal, A., Lee, B. H., Irvine, S. A., An, J., Bhuthalingam, R., Singh, V., et al. (2015). Smooth Muscle Cell Alignment and Phenotype Control by Melt Spun Polycaprolactone Fibers for Seeding of Tissue Engineered Blood Vessels. International Journal of Biomaterials, 2015, 434876-.
Series/Report no.: International Journal of Biomaterials
Abstract: A method has been developed to induce and retain a contractile phenotype for vascular smooth muscle cells, as the first step towards the development of a biomimetic blood vessel construct with minimal compliance mismatch. Melt spun PCL fibers were deposited on a mandrel to form aligned fibers of 10 μm in diameter. The fibers were bonded into aligned arrangement through dip coating in chitosan solution. This formed a surface of parallel grooves, 10 μm deep by 10 μm across, presenting a surface layer of chitosan to promote cell surface interactions. The aligned fiber surface was used to culture cells present in the vascular wall, in particular fibroblasts and smooth muscle cells. This topography induced “surface guidance” over the orientation of the cells, which adopted an elongated spindle-like morphology, whereas cells on the unpatterned control surface did not show such orientation, assuming more rhomboid shapes. The preservation of VSMC contractile phenotype on the aligned scaffold was demonstrated by the retention of α-SMA expression after several days of culture. The effect was assessed on a prototype vascular graft prosthesis fabricated from polylactide caprolactone; VSMCs aligned longitudinally along a fiberless tube, whereas, for the aligned fiber coated tubes, the VSMCs aligned in the required circumferential orientation.
URI: https://hdl.handle.net/10356/81091
http://hdl.handle.net/10220/39072
ISSN: 1687-8787
DOI: 10.1155/2015/434876
Rights: © 2015 Animesh Agrawal et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:MAE Journal Articles
MSE Journal Articles
SBS Journal Articles

SCOPUSTM   
Citations 10

21
Updated on Jun 18, 2020

PublonsTM
Citations 10

22
Updated on Mar 4, 2021

Page view(s)

221
Updated on Jul 30, 2021

Download(s) 20

133
Updated on Jul 30, 2021

Google ScholarTM

Check

Altmetric


Plumx

Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.