Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/81110
Title: Solvent-Assisted Lipid Bilayer Formation on Silicon Dioxide and Gold
Authors: Tabaei, Seyed Ruhollah
Choi, Jae-Hyeok
Goh, Haw Zan
Zhdanov, Vladimir P.
Cho, Nam-Joon
Keywords: Chemical and Biomedical Engineering
Materials Science and Engineering
Issue Date: 2014
Source: Tabaei, S. R., Choi, J.-H., Goh, H. Z., Zhdanov, V. P., & Cho, N.-J. (2014). Solvent-Assisted Lipid Bilayer Formation on Silicon Dioxide and Gold. Langmuir, 30(34), 10363-10373.
Series/Report no.: Langmuir
Abstract: Planar lipid bilayers on solid supports mimic the fundamental structure of biological membranes and can be investigated using a wide range of surface-sensitive techniques. Despite these advantages, planar bilayer fabrication is challenging, and there are no simple universal methods to form such bilayers on diverse material substrates. One of the novel methods recently proposed and proven to form a planar bilayer on silicon dioxide involves lipid deposition in organic solvent and solvent exchange to influence the phase of adsorbed lipids. To scrutinize the specifics of this solvent-assisted lipid bilayer (SALB) formation method and clarify the limits of its applicability, we have developed a simplified, continuous solvent-exchange version to form planar bilayers on silicon dioxide, gold, and alkanethiol-coated gold (in the latter case, a lipid monolayer is formed to yield a hybrid bilayer) and varied the type of organic solvent and rate of solvent exchange. By tracking the SALB formation process with simultaneous quartz crystal microbalance–dissipation (QCM-D) and ellipsometry, it was determined that the acoustic, optical, and hydration masses along with the acoustic and optical thicknesses, measured at the end of the process, are comparable to those observed by employing conventional fabrication methods (e.g., vesicle fusion). As shown by QCM-D measurements, the obtained planar bilayers are highly resistant to protein adsorption, and several, but not all, water-miscible organic solvents could be successfully used in the SALB procedure, with isopropanol yielding particularly high-quality bilayers. In addition, fluorescence recovery after photobleaching (FRAP) measurements demonstrated that the coefficient of lateral lipid diffusion in the fabricated bilayers corresponds to that measured earlier in the planar bilayers formed by vesicle fusion. With increasing rate of solvent exchange, it was also observed that the bilayer became incomplete and a phenomenological model was developed in order to explain this feature. The results obtained allowed us to clarify and discriminate likely steps of the SALB formation process as well as determine the corresponding influence of organic solvent type and flow conditions on these steps. Taken together, the findings demonstrate that the SALB formation method can be adapted to a continuous solvent-exchange procedure that is technically minimal, quick, and efficient to form planar bilayers on solid supports.
URI: https://hdl.handle.net/10356/81110
http://hdl.handle.net/10220/40642
ISSN: 0743-7463
DOI: 10.1021/la501534f
Rights: © 2014 American Chemical Society.
Fulltext Permission: none
Fulltext Availability: No Fulltext
Appears in Collections:MSE Journal Articles
SCBE Journal Articles

SCOPUSTM   
Citations

83
checked on Jul 13, 2020

WEB OF SCIENCETM
Citations

79
checked on Oct 14, 2020

Page view(s)

243
checked on Oct 20, 2020

Google ScholarTM

Check

Altmetric


Plumx

Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.