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Title: Determination of the archaeal and bacterial communities in two-phase and single-stage anaerobic systems by 454 pyrosequencing
Authors: Maspolim, Yogananda
Zhou, Yan
Guo, Chenghong
Xiao, Keke
Ng, Wun Jern
Keywords: Methanogen
Sewage sludge
2-Phase anaerobic digestion
Microbial community
454 pyrosequencing
Issue Date: 2015
Source: Maspolim, Y., Zhou, Y., Guo, C., Xiao, K., & Ng, W. J. (2015). Determination of the archaeal and bacterial communities in two-phase and single-stage anaerobic systems by 454 pyrosequencing. Journal of Environmental Sciences, 36, 121-129.
Series/Report no.: Journal of Environmental Sciences
Abstract: 2-Phase anaerobic digestion (AD), where the acidogenic phase was operated at 2 day hydraulic retention time (HRT) and the methanogenic phase at 10 days HRT, had been evaluated to determine if it could provide higher organic reduction and methane production than the conventional single-stage AD (also operated at 12 days HRT). 454 pyrosequencing was performed to determine and compare the microbial communities. The acidogenic reactor of the 2-phase system yielded a unique bacterial community of the lowest richness and diversity, while bacterial profiles of the methanogenic reactor closely followed the single-stage reactor. All reactors were predominated by hydrogenotrophic methanogens, mainly Methanolinea. Unusually, the acidogenic reactor contributed up to 24% of total methane production in the 2-phase system. This could be explained by the presence of Methanosarcina and Methanobrevibacter, and their activities could also help regulate reactor alkalinity during high loading conditions through carbon dioxide production. The enrichment of hydrolytic and acidogenic Porphyromonadaceae, Prevotellaceae, Ruminococcaceae and unclassified Bacteroidetes in the acidogenic reactor would have contributed to the improved sludge volatile solids degradation, and ultimately the overall 2-phase system's performance. Syntrophic acetogenic microorganisms were absent in the acidogenic reactor but present in the downstream methanogenic reactor, indicating the retention of various metabolic pathways also found in a single-stage system. The determination of key microorganisms further expands our understanding of the complex biological functions in AD process.
ISSN: 1001-0742
DOI: 10.1016/j.jes.2015.02.017
Schools: School of Civil and Environmental Engineering 
Research Centres: Nanyang Environment and Water Research Institute 
Rights: © 2015 The Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences. This paper was published in Journal of Environmental Sciences and is made available as an electronic reprint (preprint) with permission of The Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences. The published version is available at: []. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law.
Fulltext Permission: open
Fulltext Availability: With Fulltext
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