Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/81148
Full metadata record
DC FieldValueLanguage
dc.contributor.authorKathawala, Mustafa Hussainen
dc.contributor.authorNg, Kee Woeien
dc.contributor.authorLoo, Say Chye Joachimen
dc.date.accessioned2016-06-13T06:04:45Zen
dc.date.accessioned2019-12-06T14:22:26Z-
dc.date.available2016-06-13T06:04:45Zen
dc.date.available2019-12-06T14:22:26Z-
dc.date.copyright2015en
dc.date.issued2015en
dc.identifier.citationKathawala, M. H., Ng. K. W., & Loo, S. C. J. (2015). TiO2 nanoparticles alleviate toxicity by reducing free Zn2+ ion in human primary epidermal keratinocytes exposed to ZnO nanoparticles. Journal of Nanoparticle Research, 17(263).en
dc.identifier.issn1388-0764en
dc.identifier.urihttps://hdl.handle.net/10356/81148-
dc.description.abstractNanoparticles have been a subject of intense safety screenings due to their influx in various applications. Although recent studies have reported on the plausible cytotoxicity of nanoparticles, many of these focused only on single-material nanoparticles, while the cytotoxicity of dual-nanoparticle systems (e.g., ZnO with TiO2) has remained unexplored. For example, commercial products like sunscreens and cosmetics contain both nano-sized ZnO and TiO2, but cytotoxicity studies of such systems are meager. In this paper, the cytotoxicity of this dual-nanoparticle system comprising both ZnO and TiO2 was evaluated in vitro on skin-mimicking human primary epidermal keratinocytes (HPEKs). Inductively coupled plasma mass spectrometry, flow cytometry, and confocal microscopy were used to investigate the uptake of nanoparticles and free ions. Results revealed that ZnO nanoparticles were partially soluble (up to 20 μg ml−1 after 1 day) and could induce strong cytotoxicity as compared to the insoluble TiO2 nanoparticles which remained non-toxic until very high concentrations. It was found that TiO2 nanoparticles could play “vigilante” by protecting keratinocytes from acute toxicity of ZnO nanoparticles. This is in agreement with the observation that TiO2 nanoparticles caused an attenuation of free intracellular Zn2+ ions concentration, by adsorbing and immobilizing free Zn2+ ions. This study reveals a unique dual-nanoparticle observation in vitro on HPEKs, and highlights the importance of dual-nanoparticulate toxicity studies, especially in applications where more than one nanoparticle material-type is present.en
dc.description.sponsorshipMOH (Min. of Health, S’pore)en
dc.language.isoenen
dc.relation.ispartofseriesJournal of Nanoparticle Researchen
dc.rights© 2015 Springer.en
dc.subjectSkinen
dc.subjectSunscreensen
dc.subjectZinc ionsen
dc.subjectCellular uptakeen
dc.subjectDual particleen
dc.subjectEnvironmental and health effectsen
dc.subjectNanotoxicityen
dc.titleTiO2 nanoparticles alleviate toxicity by reducing free Zn2+ ion in human primary epidermal keratinocytes exposed to ZnO nanoparticlesen
dc.typeJournal Articleen
dc.contributor.schoolSchool of Materials Science & Engineeringen
dc.contributor.organizationSingapore Centre for Environmental Life Sciences Engineeringen
dc.identifier.doi10.1007/s11051-015-3068-4en
dc.identifier.rims194509en
item.fulltextNo Fulltext-
item.grantfulltextnone-
Appears in Collections:MSE Journal Articles
SCELSE Journal Articles

SCOPUSTM   
Citations 20

9
Updated on Jul 11, 2024

Web of ScienceTM
Citations 20

6
Updated on Oct 30, 2023

Page view(s) 20

703
Updated on Jul 19, 2024

Google ScholarTM

Check

Altmetric


Plumx

Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.