Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/81217
Title: Design and Construction of Artificial Extracellular Matrix (aECM) Proteins from Escherichia coli for Skin Tissue Engineering
Authors: Low, Pearlie Si Jia
Tjin, Monica Suryana
Fong, Eileen
Keywords: Elastin-like domains
Phase transition behavior
Protein purification
Issue 100
Genetic engineering
Protein expression
Cell-binding domain
Artificial extracellular matrix proteins
Inverse transition cycling
Bioengineering
Issue Date: 2015
Source: Low, P. S. J., Tjin, M. S., & Fong, E. (2015). Design and Construction of Artificial Extracellular Matrix (aECM) Proteins from Escherichia coli for Skin Tissue Engineering. Journal of Visualized Experiments, (100), e52845-.
Series/Report no.: Journal of Visualized Experiments
Abstract: Recombinant technology is a versatile platform to create novel artificial proteins with tunable properties. For the last decade, many artificial proteins that have incorporated functional domains derived from nature (or created de novo) have been reported. In particular, artificial extracellular matrix (aECM) proteins have been developed; these aECM proteins consist of biological domains taken from fibronectin, laminins and collagens and are combined with structural domains including elastin-like repeats, silk and collagen repeats. To date, aECM proteins have been widely investigated for applications in tissue engineering and wound repair. Recently, Tjin and coworkers developed integrin-specific aECM proteins designed for promoting human skin keratinocyte attachment and propagation. In their work, the aECM proteins incorporate cell binding domains taken from fibronectin, laminin-5 and collagen IV, as well as flanking elastin-like repeats. They demonstrated that the aECM proteins developed in their work were promising candidates for use as substrates in artificial skin. Here, we outline the design and construction of such aECM proteins as well as their purification process using the thermo-responsive characteristics of elastin.
URI: https://hdl.handle.net/10356/81217
http://hdl.handle.net/10220/39788
ISSN: 1940-087X
DOI: 10.3791/52845
Rights: © 2015 Journal of Visualized Experiments. This paper was published in Journal of Visualized Experiments and is made available as an electronic reprint (preprint) with permission of Journal of Visualized Experiments. The published version is available at: [http://dx.doi.org/10.3791/52845]. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:MSE Journal Articles

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