Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/81399
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dc.contributor.authorHo, Steven C. L.en
dc.contributor.authorYang, Yuanshengen
dc.date.accessioned2016-06-23T07:38:24Zen
dc.date.accessioned2019-12-06T14:30:06Z-
dc.date.available2016-06-23T07:38:24Zen
dc.date.available2019-12-06T14:30:06Z-
dc.date.issued2014en
dc.identifier.citationHo, S. C. L., & Yang, Y. (2014). Identifying and engineering promoters for high level and sustainable therapeutic recombinant protein production in cultured mammalian cells. Biotechnology Letters, 36(8), 1569-1579.en
dc.identifier.issn0141-5492en
dc.identifier.urihttps://hdl.handle.net/10356/81399-
dc.description.abstractPromoters are essential on plasmid vectors to initiate transcription of the transgenes when generating therapeutic recombinant proteins expressing mammalian cell lines. High and sustained levels of gene expression are desired during therapeutic protein production while gene expression is useful for cell engineering. As many finely controlled promoters exhibit cell and product specificity, new promoters need to be identified, optimized and carefully evaluated before use. Suitable promoters can be identified using techniques ranging from simple molecular biology methods to modern high-throughput omics screenings. Promoter engineering is often required after identification to either obtain high and sustained expression or to provide a wider range of gene expression. This review discusses some of the available methods to identify and engineer promoters for therapeutic recombinant protein expression in mammalian cells.en
dc.description.sponsorshipASTAR (Agency for Sci., Tech. and Research, S’pore)en
dc.format.extent65 p.en
dc.language.isoenen
dc.relation.ispartofseriesBiotechnology Lettersen
dc.rights© 2014 Springer. This is the author created version of a work that has been peer reviewed and accepted for publication by Biotechnology Letters, Springer. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1007/s10529-014-1523-4].en
dc.subjectPromoter identificationen
dc.subjectRecombinant protein productionen
dc.subjectMammalian cellsen
dc.subjectPromoter engineeringen
dc.titleIdentifying and engineering promoters for high level and sustainable therapeutic recombinant protein production in cultured mammalian cellsen
dc.typeJournal Articleen
dc.contributor.schoolSchool of Chemical and Biomedical Engineeringen
dc.identifier.doi10.1007/s10529-014-1523-4en
dc.description.versionAccepted versionen
item.grantfulltextopen-
item.fulltextWith Fulltext-
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