Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/81399
Title: Identifying and engineering promoters for high level and sustainable therapeutic recombinant protein production in cultured mammalian cells
Authors: Ho, Steven C. L.
Yang, Yuansheng
Keywords: Promoter identification
Recombinant protein production
Mammalian cells
Promoter engineering
Issue Date: 2014
Source: Ho, S. C. L., & Yang, Y. (2014). Identifying and engineering promoters for high level and sustainable therapeutic recombinant protein production in cultured mammalian cells. Biotechnology Letters, 36(8), 1569-1579.
Series/Report no.: Biotechnology Letters
Abstract: Promoters are essential on plasmid vectors to initiate transcription of the transgenes when generating therapeutic recombinant proteins expressing mammalian cell lines. High and sustained levels of gene expression are desired during therapeutic protein production while gene expression is useful for cell engineering. As many finely controlled promoters exhibit cell and product specificity, new promoters need to be identified, optimized and carefully evaluated before use. Suitable promoters can be identified using techniques ranging from simple molecular biology methods to modern high-throughput omics screenings. Promoter engineering is often required after identification to either obtain high and sustained expression or to provide a wider range of gene expression. This review discusses some of the available methods to identify and engineer promoters for therapeutic recombinant protein expression in mammalian cells.
URI: https://hdl.handle.net/10356/81399
http://hdl.handle.net/10220/40783
ISSN: 0141-5492
DOI: 10.1007/s10529-014-1523-4
Rights: © 2014 Springer. This is the author created version of a work that has been peer reviewed and accepted for publication by Biotechnology Letters, Springer. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1007/s10529-014-1523-4].
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SCBE Journal Articles

SCOPUSTM   
Citations 20

15
Updated on Jul 7, 2022

PublonsTM
Citations 20

14
Updated on Jul 9, 2022

Page view(s)

335
Updated on Aug 14, 2022

Download(s) 20

231
Updated on Aug 14, 2022

Google ScholarTM

Check

Altmetric


Plumx

Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.