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Title: Cytoplasmic long noncoding RNAs are frequently bound to and degraded at ribosomes in human cells
Authors: Carlevaro-Fita, Joana
Rahim, Anisa
Guigó, Roderic
Johnson, Rory
Vardy, Leah Karen Anne
Keywords: Cytoplasm
Transposable element
Ribosome profiling
Long noncoding RNA
Issue Date: 2016
Source: Carlevaro-Fita, J., Rahim, A., Guigó, R., Vardy, L. A., & Johnson, R. (2016). Cytoplasmic long noncoding RNAs are frequently bound to and degraded at ribosomes in human cells. RNA, 22(6), 867-882.
Series/Report no.: RNA
Abstract: Recent footprinting studies have made the surprising observation that long noncoding RNAs (lncRNAs) physically interact with ribosomes. However, these findings remain controversial, and the overall proportion of cytoplasmic lncRNAs involved is unknown. Here we make a global, absolute estimate of the cytoplasmic and ribosome-associated population of stringently filtered lncRNAs in a human cell line using polysome profiling coupled to spike-in normalized microarray analysis. Fifty-four percent of expressed lncRNAs are detected in the cytoplasm. The majority of these (70%) have >50% of their cytoplasmic copies associated with polysomal fractions. These interactions are lost upon disruption of ribosomes by puromycin. Polysomal lncRNAs are distinguished by a number of 5′ mRNA-like features, including capping and 5′UTR length. On the other hand, nonpolysomal “free cytoplasmic” lncRNAs have more conserved promoters and a wider range of expression across cell types. Exons of polysomal lncRNAs are depleted of endogenous retroviral insertions, suggesting a role for repetitive elements in lncRNA localization. Finally, we show that blocking of ribosomal elongation results in stabilization of many associated lncRNAs. Together these findings suggest that the ribosome is the default destination for the majority of cytoplasmic long noncoding RNAs and may play a role in their degradation.
ISSN: 1355-8382
DOI: 10.1261/rna.053561.115
Schools: School of Biological Sciences 
Rights: © 2016 Carlevaro-Fita et al. This article, published in RNA, is available under a Creative Commons License (Attribution 4.0 International), as described at
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SBS Journal Articles

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