Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/81506
Title: Membrane feeding of dengue patient’s blood as a substitute for direct skin feeding in studying Aedes-dengue virus interaction
Authors: Tan, Cheong-Huat
Wong, Pei-Sze Jeslyn
Li, Mei-Zhi Irene
Yang, Hui-Ting
Chong, Chee-Seng
Lee, Linda K.
Yuan, Shi
Leo, Yee-Sin
Ng, Lee-Ching
Lye, David C.
Keywords: Dengue
Membrane Feeding Assay (MFA)
EDTA blood
Aedes aegypti
Direct Skin Feeding Assay (DFSA)
Issue Date: 2016
Source: Tan, C. H., Wong, P. S. J., Li, M. Z. I., Yang, H. T., Chong, C. S., Lee, L. K., et al. (2016). Membrane feeding of dengue patient’s blood as a substitute for direct skin feeding in studying Aedes-dengue virus interaction. Parasites & Vectors, 9, 211-.
Series/Report no.: Parasites & Vectors
Abstract: Background: Understanding the interaction between Aedes vectors and dengue viruses (DENV) has significant implications in determining the transmission dynamics of dengue. The absence of an animal model and ethical concerns regarding direct feeding of mosquitoes on patients has resulted in most infection studies using blood meals spiked with laboratory-cultured DENV. Data obtained from such studies may not reflect the natural human-mosquito transmission scenario. This study explored the potential of using membrane feeding of dengue patient’s blood as a substitute for direct skin feeding.
URI: https://hdl.handle.net/10356/81506
http://hdl.handle.net/10220/40807
ISSN: 1756-3305
DOI: 10.1186/s13071-016-1469-6
Schools: School of Biological Sciences 
Rights: © 2016 Tan et al. Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SBS Journal Articles

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