Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/81537
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dc.contributor.authorChuah, Yon Jinen
dc.contributor.authorKoh, Yi Tingen
dc.contributor.authorLim, Kaiyangen
dc.contributor.authorMenon, Nishanth V.en
dc.contributor.authorWu, Yingnanen
dc.contributor.authorKang, Yuejunen
dc.date.accessioned2016-01-06T02:28:16Zen
dc.date.accessioned2019-12-06T14:33:13Z-
dc.date.available2016-01-06T02:28:16Zen
dc.date.available2019-12-06T14:33:13Z-
dc.date.issued2015en
dc.identifier.citationChuah, Y. J., Koh, Y. T., Lim, K., Menon, N. V., Wu, Y., & Kang, Y. (2015). Simple surface engineering of polydimethylsiloxane with polydopamine for stabilized mesenchymal stem cell adhesion and multipotency. Scientific Reports, 5, 18162-.en
dc.identifier.issn2045-2322en
dc.identifier.urihttps://hdl.handle.net/10356/81537-
dc.description.abstractPolydimethylsiloxane (PDMS) has been extensively exploited to study stem cell physiology in the field of mechanobiology and microfluidic chips due to their transparency, low cost and ease of fabrication. However, its intrinsic high hydrophobicity renders a surface incompatible for prolonged cell adhesion and proliferation. Plasma-treated or protein-coated PDMS shows some improvement but these strategies are often short-lived with either cell aggregates formation or cell sheet dissociation. Recently, chemical functionalization of PDMS surfaces has proved to be able to stabilize long-term culture but the chemicals and procedures involved are not user- and eco-friendly. Herein, we aim to tailor greener and biocompatible PDMS surfaces by developing a one-step bio-inspired polydopamine coating strategy to stabilize long-term bone marrow stromal cell culture on PDMS substrates. Characterization of the polydopamine-coated PDMS surfaces has revealed changes in surface wettability and presence of hydroxyl and secondary amines as compared to uncoated surfaces. These changes in PDMS surface profile contribute to the stability in BMSCs adhesion, proliferation and multipotency. This simple methodology can significantly enhance the biocompatibility of PDMS-based microfluidic devices for long-term cell analysis or mechanobiological studies.en
dc.description.sponsorshipMOE (Min. of Education, S’pore)en
dc.format.extent12 p.en
dc.language.isoenen
dc.relation.ispartofseriesScientific Reportsen
dc.rightsThis work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/en
dc.subjectBiomaterials-cellsen
dc.subjectLab-on-a-chipen
dc.titleSimple surface engineering of polydimethylsiloxane with polydopamine for stabilized mesenchymal stem cell adhesion and multipotencyen
dc.typeJournal Articleen
dc.contributor.schoolSchool of Chemical and Biomedical Engineeringen
dc.identifier.doi10.1038/srep18162en
dc.description.versionPublished versionen
dc.identifier.pmid26647719-
item.fulltextWith Fulltext-
item.grantfulltextopen-
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