Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/81542
Title: Kindlin-3 interacts with the ribosome and regulates c-Myc expression required for proliferation of chronic myeloid leukemia cells
Authors: Qu, Jing
Ero, Rya
Feng, Chen
Ong, Li-Teng
Tan, Hui-Foon
Lee, Hui-Shan
Ismail, Muhammad HB
Bu, Wen-Ting
Nama, Srikanth
Sampath, Prabha
Gao, Yong-Gui
Tan, Suet-Mien
Keywords: Extracellular matrix; Integrins
Issue Date: 2015
Source: Qu, J., Ero, R., Feng, C., Ong, L.-T., Tan, H.-F., Lee, H.-S., et al. (2015). Kindlin-3 interacts with the ribosome and regulates c-Myc expression required for proliferation of chronic myeloid leukemia cells. Scientific Reports, 5, 18491-.
Series/Report no.: Scientific Reports
Abstract: Kindlins are FERM-containing cytoplasmic proteins that regulate integrin-mediated cell-cell and cell-extracellular matrix (ECM) attachments. Kindlin-3 is expressed in hematopoietic cells, platelets, and endothelial cells. Studies have shown that kindlin-3 stabilizes cell adhesion mediated by ß1, ß2, and ß3 integrins. Apart from integrin cytoplasmic tails, kindlins are known to interact with other cytoplasmic proteins. Here we demonstrate that kindlin-3 can associate with ribosome via the receptor for activated-C kinase 1 (RACK1) scaffold protein based on immunoprecipitation, ribosome binding, and proximity ligation assays. We show that kindlin-3 regulates c-Myc protein expression in the human chronic myeloid leukemia cell line K562. Cell proliferation was reduced following siRNA reduction of kindlin-3 expression and a significant reduction in tumor mass was observed in xenograft experiments. Mechanistically, kindlin-3 is involved in integrin α5ß1-Akt-mTOR-p70S6K signaling; however, its regulation of c-Myc protein expression could be independent of this signaling axis.
URI: https://hdl.handle.net/10356/81542
http://hdl.handle.net/10220/39588
ISSN: 2045-2322
DOI: 10.1038/srep18491
Rights: This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SBS Journal Articles

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