Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/81546
Title: Genus-Wide Comparative Genomics of Malassezia Delineates Its Phylogeny, Physiology, and Niche Adaptation on Human Skin
Authors: Wu, Guangxi
Zhao, He
Rajapakse, Menaka Priyadarsani
Wong, Wing Cheong
Xu, Jun
Saunders, Charles W.
Reeder, Nancy L.
Reilman, Raymond A.
Scheynius, Annika
Sun, Sheng
Billmyre, Blake Robert
Li, Wenjun
Averette, Anna Floyd
Mieczkowski, Piotr
Heitman, Joseph
Theelen, Bart
Schröder, Markus S.
De Sessions, Paola Florez
Butler, Geraldine
Maurer-Stroh, Sebastian
Boekhout, Teun
Nagarajan, Niranjan
Dawson, Thomas L.
Li, Chenhao
Keywords: Peptidase
Malassezia
Issue Date: 2015
Source: Wu, G., Zhao, H., Li, C., Rajapakse, M. P., Wong, W. C., Xu, J., et al. (2015). Genus-Wide Comparative Genomics of Malassezia Delineates Its Phylogeny, Physiology, and Niche Adaptation on Human Skin. PLOS Genetics, 11(11), e1005614-.
Series/Report no.: PLOS Genetics
Abstract: Malassezia is a unique lipophilic genus in class Malasseziomycetes in Ustilaginomycotina, (Basidiomycota, fungi) that otherwise consists almost exclusively of plant pathogens. Malassezia are typically isolated from warm-blooded animals, are dominant members of the human skin mycobiome and are associated with common skin disorders. To characterize the genetic basis of the unique phenotypes of Malassezia spp. we sequenced the genomes of all 14 accepted species and used comparative genomics against a broad panel of fungal genomes to comprehensively identify distinct features that define the Malassezia gene repertoire: gene gain and loss selection signatures and lineage-specific gene family expansions. Our analysis revealed key gene gain events (64) with a single gene conserved across all Malassezia but absent in all other sequenced Basidiomycota. These likely horizontally transferred genes provide intriguing gain-of-function events and prime candidates to explain the emergence of Malassezia. A larger set of genes (741) were lost, with enrichment for glycosyl hydrolases and carbohydrate metabolism, concordant with adaptation to skin’s carbohydrate-deficient environment. Gene family analysis revealed extensive turnover and underlined the importance of secretory lipases, phospholipases, aspartyl proteases, and other peptidases. Combining genomic analysis with a re-evaluation of culture characteristics, we establish the likely lipid-dependence of all Malassezia. Our phylogenetic analysis sheds new light on the relationship between Malassezia and other members of Ustilaginomycotina, as well as phylogenetic lineages within the genus. Overall, our study provides a unique genomic resource for understanding Malassezia niche-specificity and potential virulence, as well as their abundance and distribution in the environment and on human skin.
URI: https://hdl.handle.net/10356/81546
http://hdl.handle.net/10220/39583
ISSN: 1553-7390
DOI: 10.1371/journal.pgen.1005614
Rights: This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SBS Journal Articles

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