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Title: Ceramides And Stress Signalling Intersect With Autophagic Defects In Neurodegenerative Drosophila blue cheese (bchs) Mutants
Authors: Papan, Cyrus
Osborne, Kathleen Amy
Torta, Federico
Shevchenko, Andrej
Schwudke, Dominik
Kraut, Rachel
Hebbar, Sarita
Sahoo, Ishtapran
Matysik, Artur
Garcia, Irene Argudo
Narayanaswamy, Pradeep
Fun, Xiu Hui
Wenk, Markus R
Keywords: Biological Sciences
Issue Date: 2015
Source: Hebbar, S., Sahoo, I., Matysik, A., Garcia, I. A., Osborne, K. A., Papan, C., et al. (2015). Ceramides And Stress Signalling Intersect With Autophagic Defects In Neurodegenerative Drosophila blue cheese (bchs) Mutants. Scientific Reports, 5, 15926-.
Series/Report no.: Scientific Reports
Abstract: Sphingolipid metabolites are involved in the regulation of autophagy, a degradative recycling process that is required to prevent neuronal degeneration. Drosophila blue cheese mutants neurodegenerate due to perturbations in autophagic flux, and consequent accumulation of ubiquitinated aggregates. Here, we demonstrate that blue cheese mutant brains exhibit an elevation in total ceramide levels; surprisingly, however, degeneration is ameliorated when the pool of available ceramides is further increased, and exacerbated when ceramide levels are decreased by altering sphingolipid catabolism or blocking de novo synthesis. Exogenous ceramide is seen to accumulate in autophagosomes, which are fewer in number and show less efficient clearance in blue cheese mutant neurons. Sphingolipid metabolism is also shifted away from salvage toward de novo pathways, while pro-growth Akt and MAP pathways are down-regulated, and ER stress is increased. All these defects are reversed under genetic rescue conditions that increase ceramide generation from salvage pathways. This constellation of effects suggests a possible mechanism whereby the observed deficit in a potentially ceramide-releasing autophagic pathway impedes survival signaling and exacerbates neuronal death.
ISSN: 2045-2322
DOI: 10.1038/srep15926
Rights: © 2015 Hebbar, S. et al. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit
Fulltext Permission: open
Fulltext Availability: With Fulltext
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