Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/81550
Title: DDX5/p68 RNA helicase expression is essential for initiating adipogenesis
Authors: Ramanathan, Nardev
Lim, Nicole
Stewart, Colin Lawson
Keywords: DDX5/p68; Adipogenesis; RNA helicase
Issue Date: 2015
Source: Ramanathan, N., Lim, N., & Stewart, C. L. (2015). DDX5/p68 RNA helicase expression is essential for initiating adipogenesis. Lipids in Health and Disease, 14, 160-.
Series/Report no.: Lipids in Health and Disease
Abstract: Background: DDX5/p68 RNA helicase is a member of the DEAD (Asp-Glu-Ala-Asp) box proteins. Apart from RNA unwinding, DDX5 is an important transcriptional factor and co-activator in cell proliferation and differentiation. Findings: Here, we have characterised the role of DDX5 in adipogenesis in 3T3-L1 cells using siRNA mediated suppression. Transient inhibition of Ddx5 mRNA expression at the start of adipogenesis impairs the differentiation programme even when DDX5 expression is restored later in adipogenesis. However transient suppression of Ddx5 at the later stages of adipogenesis do not impair adipogenesis or triglyceride accumulation suggesting Ddx5 expression is dispensable in a mature adipocyte. Conclusion: These results implicate DDX5 as a crucial factor involved in the complex transcriptional cascade of events that regulate adipogenesis and essential to the initiation of adipogenesis.
URI: https://hdl.handle.net/10356/81550
http://hdl.handle.net/10220/39564
ISSN: 1476-511X
DOI: 10.1186/s12944-015-0163-6
Schools: School of Biological Sciences 
Rights: © 2015 Ramanathan et al. Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SBS Journal Articles

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