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Title: Inducing ischemia-reperfusion injury in the mouse ear skin for intravital multiphoton imaging of immune responses
Authors: Goh, Chi Ching
Li, Jackson LiangYao
Becker, David
Weninger, Wolfgang
Angeli, Veronique
Ng, Lai Guan
Keywords: Medicine
Issue Date: 2016
Source: Goh, C. C., Li, J. L., Becker, D., Weninger, W., Angeli, V., & Ng, L. G. (2016). Inducing ischemia-reperfusion injury in the mouse ear skin for intravital multiphoton imaging of immune responses, (118), e54956-. doi:10.3791/54956
Series/Report no.: Journal of Visualized Experiments
Abstract: Ischemia-reperfusion injury (IRI) occurs when there is transient hypoxia due to the obstruction of blood flow (ischemia) followed by a subsequent re-oxygenation of the tissues (reperfusion). In the skin, ischemia-reperfusion (IR) is the main contributing factor to the pathophysiology of pressure ulcers. While the cascade of events leading up to the inflammatory response has been well studied, the spatial and temporal responses of the different subsets of immune cells to an IR injury are not well understood. Existing models of IR using the clamping technique on the skin flank are highly invasive and unsuitable for studying immune responses to injury, while similar non-invasive magnet clamping studies in the skin flank are less-than-ideal for intravital imaging studies. In this protocol, we describe a robust model of non-invasive IR developed on mouse ear skin, where we aim to visualize in real-time the cellular response of immune cells after reperfusion via multiphoton intravital imaging (MP-IVM).
ISSN: 1940-087X
DOI: 10.3791/54956
Schools: School of Biological Sciences 
Lee Kong Chian School of Medicine (LKCMedicine) 
Rights: © 2016 Journal of Visualized Experiments. All rights reserved. This paper was published in Journal of Visualized Experiments and is made available with permission of Journal of Visualized Experiments.
Fulltext Permission: open
Fulltext Availability: With Fulltext
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