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Title: Impedimetric cell-based biosensor for real-time monitoring of cytopathic effects induced by dengue viruses
Authors: Cheng, Ming Soon
Lau, Suk Hiang
Chan, Kwai Peng
Toh, Chee-Seng
Chow, Vincent T.
Keywords: Cytopathic effects
Dengue virus serotypes
Issue Date: 2015
Source: Cheng, M. S., Lau, S. H., Chan, K. P., Toh, C.-S., & Chow, V. T. (2015). Impedimetric cell-based biosensor for real-time monitoring of cytopathic effects induced by dengue viruses. Biosensors and Bioelectronics, 70, 74-80.
Series/Report no.: Biosensors and Bioelectronics
Abstract: We describe an impedimetric cell-based biosensor constructed from poly-l-lysine (PLL)-modified screen-printed carbon electrode for real-time monitoring of dengue virus (DENV) infection of surface-immobilized baby hamster kidney (BHK-21) fibroblast cells. Cytopathic effects (CPE) induced by DENV-2 New Guinea C strain (including degenerative morphological changes, detachment, membrane degradation and death of host cells), were reflected by drastic decrease in impedance signal response detected as early as ~30 hours post-infection (hpi). In contrast, distinct CPE by conventional microscopy was evident only at ~72 hpi at the corresponding multiplicity of infection (MOI) of 10. A parameter that describes the kinetics of cytopathogenesis, CIT50, which refers to the time taken for 50% reduction in impedance signal response, revealed an inverse linear relationship with virus titer and MOI. CIT50 values were also delayed by 31.5 h for each order of magnitude decrease in MOI. Therefore, based on the analysis of CIT50, the virus titer of a given sample can be determined from the measured impedance signal response. Furthermore, consistent impedance results were also obtained with clinical isolates of the four DENV serotypes verified by RT-PCR and cycle sequencing. This impedimetric cell-based biosensor represents a label-free and continuous approach for the dynamic measurement of cellular responses toward DENV infection, and for detecting the presence of infectious viral particles.
ISSN: 0956-5663
DOI: 10.1016/j.bios.2015.03.018
Rights: © 2015 Elsevier.
Fulltext Permission: none
Fulltext Availability: No Fulltext
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